Indole

First the SIM test showed that the unknown is motile but is negative for sulfide and indole production. Next, I concluded that my unknown has the ability to reduce nitrate into nitrite using nitrate reductase. Thirdly, the milk agar plate T-streak remained white meaning that the unknown lacks proteases which convert casein into a transparent…

In medical or science field, biochemical test are used to identify unknown bacteria. In this case three test are essential to identify the unknown #63 and they are MAC agar test, BCP (lactose) broth and SIM deep medium/slant. Methods such as streak plate technique, aseptic technique, and proper handling of the microbe are necessary to apply when conducting these biochemical test. Contamination during the unknown lab project shows carelessness and may put patient in jeopardy due to incorrect…

Staphylococcus epidermidis is a gram-positive cocci, that measured approximately 0.5um and had an optimum growth temperature of 37°C. Generally, when given an unknown solution, Staphylococcus epidermidis has features that can distinguish it from other forms of bacteria. After doing a gram stain, it was noticed that the bacteria had a cocci morphology, meaning that the cell can either be staphylococcus, enterococcus, or micrococcus. Using the gram stain, it can also be seen that the bacteria in…

a recent episode, she and Chef Erza help a group of active, middle aged women change their processed and high fat food eating habits. Julie informs us that the cauliflower used in one of the recipes for the ladies. provides “ an amazing amount of indole-3-carbinol for breast cancer prevention.” Cruciferous veggie are perfect for regulating insulin and…

butane-2,3-diol and indole-3-acetic acid can be carried out (35). The yeast extract solution under visible light act as reducing and capping agents. The reduction of noble metal ions to nanoparticles catalyzed through oxidation of glucose to gluconic acid by providing electrons (36, 37). The formed nanoparticles can be also stabilized by the presence of Van der Waals forces between the negatively charged oxygen groups present in the molecular structure of the butane-2,3-diol or indole-3-acetic…

The Identification of Two Unknown Bacteria Mixtures Through the Method of Characteristic Testing and Elimination Khoa Vuong TA: Charlie Roll Micro 3301 4/11/24. Abstract: There is a surplus amount of microorganisms that are out there in the world. A single swab of any surface, including the human body, will yield many different types of microorganisms. Once grown on a nutrient-rich medium, the identification of these microorganisms can be made by isolating pure colonies and performing and using…

After receiving unknown organism #14, many tests were performed to discover the identity. After running a variety of tests, my results led me to believe that my unknown organism was Klebsiella pneumoniae. K. pneumonia produced a negative-rod gram stain. My organism, K. pneumoniae, tested positive for the three main carbohydrates, glucose, lactose, and sucrose. After running tests on these carbohydrates, a pH indicator color change from red to yellow signified a positive result, while remaining…

pestis One of the five bacterium I identified was Yersinia pestis. It is a gram negative rod and these are the tests that identified it (and the results of those tests): gram morphology (g-rod), oxidase (negative), lactose fermentation (negative), indole (negative), urease (negative), motility (negative), orthine dicarboxylase (negative). These tests were chosen as directed by my flow chart. Discovery During a plague epidemic in Hong Kong in 1894, Yersinia pestis was discovered by the…

Celsius for 48 hours, and transferred afterwards to a refrigerator for further inspection. The agar slant and deep agar tests included Oxidation-Fermentation test, LIA test, Carbohydrate tests, Gelatin Hydrolysis, Motility test, H2S Production test, Indole test,…

10-30 seconds, and Safranin for 1 minute, and then when you take a sample, examine it under a microscope. Next use MSA and Blood Agar. Use a loop to streak the sample onto agar plates and incubate. Then use the SIM tube to test for sulfur reduction, indole production, and motility by using an incubation needle to transfer…