Indole

SIM deep covers three tests, namely motility, hydrogen sulfide reduction and indole production. Using an inoculating needle, get an inoculum from one of the slants that was transferred last week and then stab the SIM deep medium for about an inch. Incubate the medium at 37˚C for about 48 hours. Citrate test was then conducted. Simmons…

The heterocyclic compounds naming tetrahydrocarbazoles having the significant biological properties. The newly substituted tetrahydrocarbazole derivatives were prepared from substituted phenylhydrazine and cyclohexanone in glacial acetic acid under reflux. These intermediates on reaction with substituted aromatic acid chlorides in alkaline media finally converted to N-Substituted tetrahydrocarbazoles. Fifteen compounds are synthesized and charaterized by their melting point (MP), IR, NMR, MS…

it was gram negative. I then continued with some tests that were specifically for gram negative bacteria. I did an OF glucose test, a Citrate test, SIM test which told me the H2S, Indole and motility results and also a urease test. After letting my tests incubate I found that there were negative results for the H2S, Indole and urease tests and positive…

Feces of patients that have symptoms of food poisoning or typhoid fever are cultured for Salmonella. Often, the preliminary diagnosis is first suggested by recognition of an outbreak (multiple patients having symptoms of a disease all at about the same time and often from the same food or water source). Again, many organisms and toxins can cause food poisoning, so it is sometimes difficult to determine the outbreak's cause. Usually, the laboratory needs to be notified that S. spp. is suspected…

Desulfovibrio is gram negative bacteria. There are media that will be useful in isolating this organism. The best option for a gram negative bacteria would be using a selective media specifically MacConkey agar or EMB agar. These agars are used to isolate gram negative bacteria because the crystal violet in the MacConkey agar will not allow the growth of gram positive bacterium making it a good media to isolate Desulfovibrio. If that selective media does not work then another media is to use a…

tested by adding the unknown specimen to a tube of Methyl Red (MRVP) broth, and the tube was incubated. A sulfide, indole production, and motility test, or SIM, was conducted by obtaining a tube of SIM agar. The agar was inoculated with the unknown by the inoculating needle. The sulfide and motility portion of the test was then concluded. After incubation at 37C for 24 hours, the indole test was…

After, then other biochemical tests were performed that were specific to the gram- negative bacteria to start the identification process of the unknown. The results of the gram negative tests lead to the identification of the bacteria Alcaligenes faecalis. The gram stain procedure was the first test performed by isolating a colony from the pure culture streak plate. The gram stain was followed orderly right from the referenced laboratory manual. The test resulted to be Gram- Negative bacteria…

Intro: A. Alkaloids are the most diverse group of natural products that exist within living organisms (Hartmann, 1998). Alkaloids are a diverse group of low molecular weight; nitrogen-containing compounds mainly derived from amino acids, and are found in about 20% of plant species. Alkaloids play a very important role in organism metabolism and functional activity. They are metabolic products in plants, animals and microorganisms (Aniszewski, 2007). Due to their potent biological activity, many…

In this lab report a number of tests and results will be discussed. Unknown labs are performed for many reasons ranging from figuring out the causative agent in a diseased patient, to knowing the correct microorganism to use when making certain foods and antibiotics. Unknown labs are a key to testing the knowledge a student has gained throughout the course. Materials: Microscope Bunsen Burner Beaker Test Tube Holder Test Tube Clothes pin Striker Loops and Needles Distilled Water Crystal…

The purpose of the unknown experiment is to understand and identify a gram positive and a gram negative. Bacteria can be very helpful in clinical uses and many pharmaceutical drugs are based on the organism. Knowing how a bacterium works and how it is structured can be crucial to determine how it affects each individual. Several biochemical tests were performing to separate and determine the identities of the microorganisms. Broth tube number 31 containing unknown microorganisms. One of the…