Indole

1. Differential diagnosis (names of possible bacteria) You should include at least 6 most relevant bacteria that can cause signs and symptoms given in the case study and rationale for including each of them (1 sentence maximum for each). An elderly man at the hospital emergency has symptoms of high temperature (at 103oF) and an above respiratory rate. Possible bacterial pathogens that could have caused his illness are: Haemophilus influenza – this bacterium, which is more susceptible to the…

This isolate did not reduce sulfur, no indole was formed and there was no motility. A FTM test was performed and this tested the aerotolerance of the microorganism. The results of this test was that the microorganism grew more towards the top of the tube. This means that the microorganisms is…

The three medium are sulfur reduction, indole production and motility. For sufur reduction, the unknown bacteria was able to reduce sulfur to hydrogen sulfide. There was a positive result for this part of the test. There were black precipitate which is a sign for a positive result. For motililty, It also test positive by the cloudy red result of the medium. The last part of the test is indole. This test the microoganism be able to produce tryptochan . The bacteria…

Introduction In this lab report I use two different techniques to identify Unknown A and Unknown B bacteria’s. These techniques are gram staining and metabolic testing. I first used Gram staining to distinguished and identify the bacteria’s. Han Christian discovered gram staining in 1882, he had biopsy a patient lung that had pneumonia. The main purpose for Gram staining is to identify if is Gram positive or Gram negative by using differential staining. While Gram staining shows the…

on results gather unknown 5 was identified as Enterobacter aerogenes because the methyl red test was positive and the urea test was negative. And the H2S production test was negative, leaving with only E.coli and Enterobacter aerogenes. Since the indole test was negative this eliminated E.coli and Enterobacter…

which stands for the Sulfide Indole Motility. In this experiment we prepared the agar by stabbing it with the microorganism and letting it grow. The results would tell us if our microorganism broke down certain amino acids and produces the byproducts and the growth out from the stabs to indicate the motility of the substance. After the addition of the reagent to detect the Indole production our specimen appeared to have experimented negative for hydrogen sulfide and Indole but experimented…

Abstract: The objective of these series of experiments was to identify two unknown bacteria’s. Broth culture #20 was selected and subjected to qualitative tests for identification. Gram stain tests were performed in order to identify which unknown is gram positive and gram negative. Using selective and differential media, like MacConkey agar which allows the growth of gram negative bacteria only that are able to ferment lactose. Also, mannitol agar was used which isolates and detects…

positive for decarboxylate lysine it will have a purple butt, and if negative it will have a yellow butt. The Sulfide Indole Motility (SIM) blue cap tube is a semisolid agar and it is also used to determine sulfide production, indole formation and motility in Gram negative bacteria. We will be adding a couple of drops of Kovacs reagent following the incubation of the unknown organism. The indole combines with the reagent and if positive it produces a red layer on top of the medium. If the medium…

Section 1: Tests used to identify unknown pathogen Mario F. Pedero a 30-year-old male visited his physician complaining of painful bumps and soars on his leg. The patient was diagnosed with HIV + 5 years ago. Due to his diagnosis, cutaneous disorders may manifest from the condition itself, or it can lead to opportunistic infections or infections that are more extensive than normal. The patient was sent to the emergency room were the physical examination revealed skin redness, papule lesions, and…

red reagent, and the other with Barrit’s reagent. Methyl Red test is used for the indication of fermenting ability of bacteria, producing mixed acid. Voges-Proskauer test is used to detect the production of acetoin, also known as 2,3-butanediol. 6. Indole – Motility…