Indole

amino acids cysteine and methionine are decomposed, they produce hydrogen sulfide gas. Certain bacteria produce the enzymes necessary to break down those two amino acids and a simple stab test can be done to see if the bacterium is present. A sulfite indole motility medium is utilized for this test and stabbed with inoculated bacteria. A positive result would be a black precipitate at the bottom of the medium. 5. Another test used was the urea hydrolysis test. This test utilized the…

There were many tests performed on my unknown, which led me to conclude that the unknown microorganism is Pseudomonas fluorescens. When determining the gram reaction of the unknown, a Gram stain was done. This process differentiates between a Gram-positive and Gram-negative microorganism. This is done in four steps. The microorganism was stained with iodine, crystal violet, alcohol and acetone, and safranin. Iodine was used first as it acts as a mordant. It forms an insoluble complex by binding…

I performed the same tests on bacteria two, that I had performed on bacteria one. The first one was the SIM test, my results showed that this bacteria was negative for sulfur reduction, due to the lack of black precipitate. As well as negative for indole, there was no color change. Lastly, it was negative for motility because there was no growth outside of the initial stab. I next performed the phenol red broth fermentation tests. These were only left in the incubators for twenty-four hours…

inhibitory activity, such as the triterpenoid alkaloids isolated from Buxus hyrcana (Orthan et al., 2004), the steroidal alkaloids from Buxus papillosa (Babar et al., 2006), the indole glucoalkaloids from Chimarrhis turbinata (Cardoso et al., 2004), the monoterpene alkaloids from Hippeastrum argentinum (Ortiz et al., 2016), the indole alkaloids from Rauvolfia reflexa (Fadaeinasab et al.,…

pigment as their morphology. The organisms in this genus can also be oxidase positive or negative. In the same genus, some organism can convert glucose to acid and some can’t; and some can reduce nitrate while other can’t. It lacked the result for Indole to tryptophan. Although the test for Pseudomonas are mutual to both ends (positive and negative) for most test, there is still possibility that the unknown can be from this genus; therefore, it can’t be omitted. However, this can be removed if…

With applications of chlormequat at 10 to 320 ppm to cotton plants ((3. hirsutum L. and 2° arboreum L.), 70 to 80 days old, Oosterhuis (1995) noted that plant growth was retarded and yield was significantly increased by increasing the number of bolls/plant and boll weight. The highest yield increases (18 to 45%)' in (3. hir sut um L. cult ivars occurred with a spray of 40 ppm while yield increased in G_. arboreum L. cult ivars (16 to 34%) with an application of 160 ppm. and Stewart (2005)…

Materials and method Microorganisms are classified in to the three domains of life. The organism that is predominately found in our environment is bacteria closely followed by archaea and eukaryote. Although, microorganism might seem similar, they are different interims of their structures, biochemical composition, and the habitat they adopt. Conducting an extensive test on microorganism has many benefits. We can protect our self’s from different types of infectious disease. In the unknown…

Introduction : Enterotube II is used to identify multiple tests in one tube. twelve different conventional media plus a self-contained inoculating wire. The twelve test are for glucose and gas, then lysine, ornithine, H2S and indole, adonitol, lactose, arabinose, sorbitol, dulcitol, phenylalanine (amino acid), urea, and citrate (No VP test require). These medias performs 15 different biochemical tests that will determine the genus and the species of gram negative bacteria. This test will help…

unable to ferment lactose. Klebsiella pneumonia and Enterobacter aerogenes can ferment lactose to a certain extent where as Citrobacter freundii and Escherichia coli excessively ferment lactose. In our indole test, only Escherichia coli and Proteus vulgaris are able to convert tryptophan to indole. We were able to learn a lot from the TSI agars. The Citrobacter freundii, Proteus vulgaris, and Salmonella enteritidis all utilized sulfur and thus produced H2S leaving behind a black color at the…

However, the indole test from the SIM test, citrate test, and the growth of the EMB plate was deemed to be the most important test because the unknown bacteria was tested positive for indole, negative for citrate, and had metallic green sheen, with purple and black centers on it. This indicates that unknown 18 for the gram-negative bacteria was in fact…