Indole

Purpose: To identify an unknown microorganism by performing a series of biochemical tests on a pure bacterial culture. Materials and Methods: Carbohydrate Utilization: Materials: - Phenol red lactose Broth - Phenol Red Sucrose Broth - Unknown organism # 40 - Inoculating tool Procedure: Phenol red lactose and Phenol Sucrose were cultures used to identify fermentation of carbohydrates for the unknown organism #40. Phenol red is a ph. indicator as the inverted Durham tube indicates gas production…

tryptophanase. The indole test is used to determine if the bacteria is the indole positive Escherichia coli or one of the indole negative bacterium Enterobacter or Klebsiella. This is a good test to perform specifically because it either tells you which bacterium you have or that you need to perform more tests to determine which of the other two other genus it could be. Post 37o Celsius incubation, students are to add kovac’s or Ehrlich’s reagent to the culture. Theses reagents react with indole…

The second test tube 11 was identified as a gram negative bacteria. The biochemical tests that I performed that were positive were catalase, glucose, SIM, indole, citrate, and urea. First, the catalase test has the presence of an enzyme in the test isolated detected using hydrogen peroxide. Bacteria possess catalase with a small number of bacteria; isolation is added to hydrogen peroxide when bubbles occur. When the bubbles occurred on gram positive bacterium it was a conformation of being…

Gram-positive cocci are an interesting group of bacteria to study. Some common species include Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Streptococcus pyogenes, and Sarcina lutea. Gram-positive cocci have a gram positive cell wall, cocci morphology and many different styles of grouping. Some species have begun to exhibit a resistance to fluoroquinolone by the use of altering the drug binding site and the use of a membrane pump (Hooper 2002). Thanks to the…

gram negative isolate had a negative result on the lactose test. Using this result, the unknown separation outline directed me to perform an Indole test. From the results shown in Figure 5, the lack of black precipitate indicates a negative H2S test and the lack of a red ring formed at the top of the tube after adding Kovac’s reagent indicates a negative Indole test (3). However, the bacteria did grow beyond the initial stab line, which indicates that the bacteria is motile (3).…

Biochemical Unknown I. Introduction Cultural Characteristics or morphology and biochemical tests can be used to identify and classify microorganism. By culturing microorganism in nutrient broth, slants, and on nutrient agar plates, the cultural characteristics or morphology can be determined. In this lab, the test tube 2 was incubated in a nutrient broth. The pigmentation of the tube was yellow. The media for test tube 2 was turbid. There were a few sediment on the bottom of the broth. In…

The study of microbiology requires not only understanding the microscopic organisms, but also the understanding of lab techniques and procedures used to identify, control, and manipulate microorganisms. The identification of microorganisms is not only important in microbiology lab, but also in the medical field to identify an agent of a disease that will help treat the patient by using the correct antibiotics to kill off the host. In this unknown lab report, techniques and procedures learned in…

semi-solid media contains casein, amino acids, iron-contain compound and sulfur in form of sodium thiosulfate. Inoculated by stabbing technique and incubated for 24 hours, if the organism could reduce sulfur, a black color would be seen. To test for indole production, four or five drops of Kovak’s reagent was added into the cultured, a red color indicated a positive result. Last was to test for motility , if cloudiness was seen away from the stab line, then motility was…

In order to correctly identify each organism, a chart was created from Bergey’s Manual of Determinative Bacteriology that incorporated all known biochemical test results for each of the thirteen possible organisms. The thirteen organisms were separated into three separate categories, Gram-negative bacilli, Gram-positive bacilli, and Gram-positive cocci. This table can be found in the appendix (Bergey and Holt, 1994). Based on the information in the chart, the order of biochemical tests…

The sequence of the reactions employed for the construction of novel indole–benzimidazole-based 1,2,3-triazole hybrids is demonstrated in Scheme 1. Compound 2 was prepared by treating propargyl bromide with 1H-indole-3-carbaldehyde (1) in presence of potassium carbonate in dry acetone at reflux temperature [26]. Compound 2 was further reacted by heating at reflux temperature with o-phenylenediamine (OPDA) in chloroform for 6 h afforded compound 3. Compound 3 coupled with variously substituted…