Indicator

Experiment 3 titled “Physiologically Important IONS” and the purpose of this lab is to become familiar with metal and nonmetal ions and mostly important in biological and physiological processes. Also, to become familiar with tests to determine the presence or absence of ions as well as to estimate approximate amount. To begin, when a solution is tested, make sure you observe everything and note everything. This is lab, we will test some positive ion and determines if it’s an anion or a cation.…

III. Results Table 2: KHP Vs. NaOH Titration data Trial Initial KHP acid Volume (ml) End KHP acid volume (ml) Volume difference (ml) Volume of NaOH Base Used (ml) 1 7 mL 16 mL 9 mL 10.0 mL 2 16 mL 27.5 mL 11.5 mL 10.0 mL 3 27.5 mL 38.5 mL 11 mL 10.0 mL Table 3: Titration of HCl with NaOH Trial Initial NaOH Volume (ml) End NaOH base volume (ml) Volume difference (ml) Volume of HCl acid Used (ml) 1 4.5 mL 14.5 mL 10 mL 10.0 mL 2 30 mL 41.5 mL 11.5 mL 10.0 mL 3 19 mL 31 mL 12 mL 10.0…

3.7.1. Effect of solvent and pH The influence of the solvent on Schiff base ligand L and its metal(II) complexes were studied. The results showed that the optimal solvent for Schiff base ligand L, Co(II), Ni(II) and Cu(II) complexes were CH3CN at λemi =320, 310, 316 and 330 nm, while the optimal solvent for Mn(II) complex was DMF λemi = 312 nm, as shown in Figure 7. The effect pH on the fluorescence intensity of Schiff base ligand L and its metal(II) complexes were investigated between…

Question 1: pH can be described as a measure of acidity or basicity (Alkalinity) of a solution. It is exactly equal in amount to the negative log of the concentration of hydronium(H3O+) ions. Due to the negative log relationship, increased hydronium ions transform to lower pH. When we add it to water, an acid releases hydrogen ion. Hydrogen ions in water unite with the water molecules to form hydronium ions, thereby lowering the pH and increasing the acidity of water. Water exposed to air is…

2. Materials and Methods: 2.1. Materials: Natural red, propidium iodide (PI), acridine orange and phosphate-buffered saline (PBS) were obtained from Sigma-Aldrich (St.Louis, MO). May–Grunwald–Giemsa stain was procured from Merck (Darmstadt, Germany). Fetal calf serum, Dulbecco's Modified Eagle Medium (DEMEM) and RPMI 1640 were bought from GIBCO/Life Technologies Inc. (Gaithersburg, MD). 2.2. Isolation and Proliferation of MSCs Bone marrow derived MSCs were isolated as described previously…

Observations: This experiment was divided into two parts: one part was to observe the properties of a single Alka Seltzer tablet and the second part was to find the percentage of CO2. In the first part of the experiment, the visible properties of Alka Seltzer were observed and recorded. Each Alka Seltzer contains the same three ingredients: 325 mg of Aspirin, 1000 mg of Citric Acid, and 1916 mg of Sodium Bicarbonate. Two of Alka Seltzer’s ingredients, Citric Acid and Sodium Bicarbonate (Baking…

In the biomolecule lab, we conducted the lipid chromatography experiment to separate a mixture of biomolecules from lipids. If a positive test is conducted by the end of the experiment, there should be smudges aligned vertically on the chromatography paper, or silica gel strip, indicating the separation of different lipid molecules that were present in the substance. In contrast, if a negative test is conducted there would be no smudges aligned vertically on the chromatography paper. Substances…

PH levels are a measure of how acidic or basic your beverage is. They show what beverages are more acidic, like soda, and what beverages are more basic, like water. It’s measured on a scale from one to fourteen. One through six are the acid levels, seven is the only neutral level, and eight through fourteen are the basic levels. First, get 2 drinks of choice and pour them into two different cups. Then, record its pH level, as well as the room temperature. Next, take another liquid and mix it…

1. The first part of the second experiment went according to the alternative hypothesis. The 200% saline water moved below the 100% saline water to create a layer. It did not mix with the 100% saline water or tap water. On the other hand, the second part of the second experiment did not support the alternative. Instead of moving above the 100% and 200% saline water and tap water, the 35% saline water remained at the bottom. However, it did try to move up. The reason the 35% saline water did not…

At 20 seconds, pH = 7.35 2. At 40 seconds, pH = 7.29 3. At 60 seconds, pH = 7.24 4. Did the pH level of the blood change at all during this run? If so, how? Yes, the pH level of the blood decreased. 5. Was the pH level always within the “normal” range for the human body? If not, when was the pH value outside of the normal range, and what acid/base imbalance did this pH value indicate? No, acidosis occurred at 40 seconds when the pH dropped out of “normal” range to…