In vivo

Calibration Plots for Raloxifene Hydrochloride were developed in Blood Plasma for in-vivo studies and Rat Liver Homogenate for ex-vivo studies, using HPLC. R2 values of both the calibration plots were nearer to 1, which indicated that the methods developed were following Beer-Lambert’s law and could be used for further estimation. Analytical Interference Study…

Intervention Outline PE is an individual therapy that is highly flexible and can be modified to fit the needs of clients from diverse backgrounds. PE is specifically designed to help clients process traumatic events and reduce trauma-induced psychological disturbances, making it one of the most effective interventions for individuals experiencing rape-related PTSD (Penn Behavioral Health, 2017). While the PE treatment program is structured, it can be modified in order to best assist an array of…

We demonstrated that both MmuPV1 E6 and E7 are oncogenic in vitro and in vivo (Preliminary Data). We have also constructed several MmuPV1 mutants (E3ATGko, L1ATGko, L2ATGko and L1+L2ATGKo, E6 and E7 codon modified genomes) that showed different phenotypes in vivo (manuscripts in preparation). In addition, we have developed an in vitro assay system to quantitate viral infectivity and neutralizing activity. Although we…

In a schwannoma tumor model, we demonstrated that genetically engineered EVs containing the suicide gene mRNA and protein-cytosine deaminase (CD) which is fused to uracil phosphoribosyltransferase (UPRT) reduced tumor growth in vivo. Glioblastoma is the most common primary brain tumor and highly aggressive with limited treatment options. Therefore we established a subcutaneous glioblastoma tumor model in mice to investigate if our approach can be used here as well. We implanted…

Skeletal muscle is a highly specialized, post-mitotic tissue that must withstand chronic mechanical and physiological stress throughout life to maintain proper contractile function. Muscle damage or disease leads to progressive weakness and disability, and manifests in more than 100 different human disorders, including DMD, the most common X-linked genetic disorder in humans, and BMD. Current treatment options for muscular dystrophies are disappointingly limited and focus mainly on managing…

Effect of thymol and carvacrol on nutrient digestibility in rams fed high or low concentrate diets Summary Published data on the effects of essential oils (EO) on in vivo nutrient digestibility in sheep are contradictory. In 2 experiments, the effect of thymol and carvacrol on nutrient digestibility was studied in sheep fed with high (70%) or low (52%) concentrate diets, using incomplete Latin Square designs. The essential oils were mixed with the concentrate portion of the diet at the rate of 0…

experiments doesn't give us the desired output since there are other neurons in the brain that acts all together in the order in a specific way to give us the bird song. b. In vivo experimental setup: In order to know how the basal ganglia projecting cortical neurons operate in vivo, a miniature micro-drive is manufactured for in vivo recording, this micro-drive is connected directly to the brain of the bird, after the removal of the head bone and the surface layer of the brain, and the HVc…

The human genome can be described as a set of genetic instructions also known as an organism’s set of DNA, which allow a human being to grow and develop. There are approximately more than 3 billion DNA pairs within an entire genome, containing millions of cells, which all carry various instructions. Inside of our DNA, there is a unique chemical code, which determines our growth, development, and health. This specific code is derived by four nucleotide bases that conform the DNA. These are called…

Critique of Systematic Reviews Two systematic review articles will be critiqued and compared in this paper. Article A is titled systematic review and meta-analysis of adverse events of low-dose aspirin and clopidogrel in randomized controlled trials authored by Kenneth McQuaid and Loren Laine. Article B is titled platelet function testing in transient ischemic attack and ischemic stroke: a comprehensive systematic review of the literature by Soon Lim, Catherine Coughlan, Stephen Murphy, Israel…

Cancer is a most ravaging disease threatening globally with a leading mortally rate in developed countries. 1 Various types of cancers have been identified which affects different organs and they are broadly grouped under the category of carcinoma, sarcoma, lymphoma or leukemia based on the type of tissue origin. common striking features of all types of cancer is uncontrolled cell proliferation, antiapoptosis, enhanced angiogenesis, genomic instability and metastasis. 4 The broad range of…