In vivo

Aim 1: Analyzing the formation of the epithelium of visceral endoderm surrounding the internal cavity in locally-instructed embryoids Sub-aim 1.1: Investigating the formation of an endodermal epithelium around the cavity Rationale: VE is derived from PrE, an epithelial tissue that separates the ICM from the blastocoel. PrE and Epiblast (Epi) cells exist in a ‘salt and pepper’ manner in the ICM by E3.5, which can be visualized by the complementary expression patterns of Gata6 (PrE) and Nanog…

BRMS1 PROMOTER AND METASTATIC BREAST CANCER BRMS1, a functional gene, is composed of 10 exons and ranged from 7.8kb of genomic DNA. It is a member of mSIN3-HDAC transcription co-repressor complex and resides normally in the nucleus. Product of alternative splicing, two mRNA transcripts, which are in turn translated into two distinct proteins, 246 amino acids and 290 amino acids. When the structure of BRMS1 promoter is studied, two hyper methylated cytosine-phosphoguanine (CpG)…

OVERVIEW Four ablations will be done in ex vivo bovine for each flow rate. There will be three different flow rates: 0 mL/min, 860 mL/min, and 1000 mL/min. These flow rate were chosen based on hepatic flow rates of humans in the supine position (Brown et al. 1989; Leen et al. 1992). Each ablation will be performed at 40 watts for 2 minutes for a total of 12 ablations. The average area of a 30ºC and 50 ºC contour at the end of the ablation will be used to characterize the size of the ablation…

The first receptor I will be doing is the Somatostatin receptor there is so much behind this receptor. Somatostatin (SST), otherwise called somatotropin discharge restraining variable (SRIF), is a hypothalamic hormone, a pancreatic hormone, and a focal and fringe neurotransmitter. Somatostatin has a wide conveyance all through the focal sensory system (CNS) and also in fringe tissues, for instance in the pituitary, pancreas and stomach. The different activities of somatostatin are intervened by…

Abstract: Curcumin is a spice that is the active ingredient in traditional foods, which has an incredible amount of beneficial properties, such as anti-inflammation and chemo preventive action. It can also act as an antioxidant and can even chelate irons. Currently, curcumin is involved in human clinical trials for many different conditions like multiple myeloma, Alzheimer’s disease, and colon cancer as a potential therapeutic agent. The purpose of this proposal is to present the current level…

Drug resistance against conventional therapies of cancers is an unfavorable phenomenon which reported by numerous authors (20-22). So, to overcome drug resistance in different cancers researchers have turned to use new tumor therapy approaches based on creating change in kinetic of immune modulators (15, 23, 24). In this regard, SEB is a potent superantigen which can deteriorate tumor cells through growth inhibition and apoptosis inducing. Furthermore, SEB plays critical roles against different…

What is UGT1A4? The majority of drugs are metabolized to form compounds that are more polar in structure than their parental precursors. The ultimate goal is to 1) increase the hydrophilicity of the presented compounds along with their molecular weight, 2) inactivate their biological effects and 3) facilitate their efflux from the cells, subsequently followed by excretion into bile or urine1. The metabolic enzymes in charge of this process are traditionally organized into two separate…

Of Mice and Men Animals are murdered, tortured, and abused every day, but it is overlooked “in the name of science.” The use of animals for experimentation can be traced back to the second century. The first person known to have performed these experiments was named Galen, also known as “the father of vivisection.” Although, the first writings were believed to have originated from the Greeks, Aristotle and Erasistratus were among the first to perform on living animals. Animal testing is not a…

1.6 Hodgkin’s Lymphoma Previous in silico research in our laboratory found that H2A.B is highly expressed in one particular cancer type, Hodgkin’s Lymphoma. Hodgkin’s Lymphoma (HL) is a type of B cell cancer that affects both men and women of various ages. HL can be divided into two classes: classical HL (cHL) and Nodular Lymphocyte Predominant HL (NLPHL) according to the WHO classification 2008 (Swerdlow et al., 2008). The classical HL accounts for 95% of all HL cases, and it has been further…

demonstrate substandard results in academic achievement scores compared to their siblings. Studies show that children with Duchenne Muscular Dystrophy have poorer verbal memory spans, self-control problems, and physical difficulties, as well. (Hinton, Vivo, and Fee,…