In vitro

Tissue engineering strategies for repairing skeletal muscle are split into the in vitro or the in vivo approach. The in vitro approach attempts to engineer mature and contractile muscle constructs by culturing cells on a biomaterial substrate until it has evolved into a functional tissue that can be transplanted into patients. The in vivo approach involves transplanting cells, either unaided, or in combination with a biomaterial scaffold, to create a local niche at the site of injury from where…

Coelogyne flaccida seed culture in vitro reveals that there are two pathways of asymbiotic seedling development from seeds. On one pathway, the seeds obtained from green capsules germinated readily asymbiotically in the nutrient medium supplemented with 0.5 – 2 mg/l NAA and IAA plus 15% coconut water and charcoal, 2 gm/l. During the usual germination process, the undifferentiated embryo of the seed produces a large number of protocorm-like bodies (PLBs) without forming the intermediate callus…

are made by bone marrow and released into the blood. The coagulation factor will also be tested more in-depth including factors VII and VIII assays. On the other hand, many drugs that are in the early phase of their discovery are tested using in-vitro hepatotoxicity testing. This is the recommended method to test the compound so one can see the molecular structure of the drug. It can be tested in a controlled environment with different variables added to make structured toxicology tests. It is…

Masci discusses how in the early years of in vitro fertilization, many feared this new technology in the same way that people disagree with genetic alterations today. As an in vitro baby myself, I can personally reveal that I am not treated a “good or commodity”. On the contrary, I believe that the difficult struggle to conceive has only increased my parents’ gratefulness…

manipulated through the use of Assisted Reproductive Technology, namely in vitro fertility treatments used on barren women. Methods may be used to assure avoiding conception, but should abortion be used to avoid an unwanted pregnancy. Abortion is taking a life where there should be life. Assisted Reproductive Technology creates life where there should be no life. If abortion is unnatural from a Christian perspective, then so too is in vitro fertility. How can Christians condone the use of ART,…

Ruby Arndt Ms. Francis English 9 27 April 2018 Silver Spring Monkeys aren’t the type of animal you hear on an everyday basis, however they are certainly important in today’s society. In 1981, Alex Pacheco went undercover as a scientist for the Institute for Behavioral Research. During his work there, he discovered 17 silver spring monkeys captured in small metal cages, all of them suffering from living in their own stench and feces. Many of the silver spring monkeys had lost the movement of…

testing is still the best way to find new treatments for patients, is simply that, animal testing being the best way to help find cures and help people. When they start off by comparing using test tubes (vitro) vs using animals (vivo). In their first argument they explain that sometimes in vitro is better than vivo in some cases. They use the example of about “isolated cancer cells [and how they] are more sensitive to an anticancer agent than in vivo because [… of] factors [that] limit the drug…

We demonstrated that both MmuPV1 E6 and E7 are oncogenic in vitro and in vivo (Preliminary Data). We have also constructed several MmuPV1 mutants (E3ATGko, L1ATGko, L2ATGko and L1+L2ATGKo, E6 and E7 codon modified genomes) that showed different phenotypes in vivo (manuscripts in preparation). In addition, we have developed an in vitro assay system to quantitate viral infectivity and neutralizing activity. Although we cannot test vaccines in the…

alternative, in-vitro research is the study of an organisms cells’ outside of its normal biological context. Essentially, scientists have discovered a method of testing molecules in a controlled environment. A major success from in-vitro testing was the polio vaccine. Researchers spent decades infecting non-human primates with the disease, but failed to produce a vaccine. However, the key event that led directly to the vaccine was when researchers grew the virus in human cell cultures in-vitro.…

In our previous report, 21 we investigated the influence of formulation variables of clotrimazole-loaded cubic liquid crystalline nanoparticles. The composition of chitosan tailored cubic nanoparticles was optimized employing central composite design. The findings of Small angle X-ray scattering analysis confirmed the presence of D and P cubic phases in nanoparticles. Most importantly, considerable improvement in mucin binding was observed upon incorporation of chitosan owing to electrostatic…