Immobilized enzyme

Methods 1. Treatment of barley with endophyte. • Five types of Barley were treated with the endophyte Pseudomonas fluorescens L321. • They were innoculated with the seed coating mixture ( 4% sodium alginate and 1% skimmed milk powder) without the bacteria. The bacteria were grown up overnight in LB broth supplemented with 3% trehalose (400ml per strain) , centrifuged and then added and then resuspended in the coating mixture. • 30ml of inoculant mixture was then added to 1kg of seed in a…

Some physical and chemical properties of watermelon rind and sharlyn melon peel powders Proximate composition WAC, OAC and antioxidant activity of watermelon rind and sharlyn melon peel powders Data in Table 2 showed that the watermelon rind powder had higher moisture, ash, fat, protein and carbohydrates (10.61%, 13.09%, 2.44%, 11.17% and 56.02%, respectively) as compared to sharlyn melon peel powder. On the other hand, the sharlyn melon peels had higher content of crud fiber (29.59%) as…

5. Protein purification There are many thousand kinds of proteins with different properties and functions in a cell. Proteins have been purified in active form on the basis of such characteristics as solubility, size, charge and specific binding affinity. Normally, protein mixtures are subjected to a series of separations, each based on a different property to yield a pure protein. 5.1 Separation based on solubility (Scopes, 1994) The protein solubility in various solvents depended on the…

In 1935, the first plasma membrane structure model was introduced by Danielli and Davson. They proposed that the membrane is made of phospholipid bilayer that consists of hydrophobic head and the hydrophilic tail. Hydrophilic head, which is polar, facing towards the water molecules and said to be coated with protein. Hydrophilic tail, which is non-polar is not facing water molecules and sandwiched between the two bilayers. At regular intervals of the surface of the plasma membrane, there are…

The effect of CKD on sludge stabilization compared with chemical stabilization with Lime is introduced in Table (3). Also comparison between different concentrations of sludge is introduced. Different dosages of CKD were examined and the dosage that achieves stabilization was 50% and 60% for thickened and diluted sludge respectively, while the required dosage of Lime was ranged between 22 and 29%. The required lime dosage complies with the results of EPA [4], Wang [18], Rahman [11] and Salah…

the rate of reaction on Enzyme reaction Introduction: This experiment will investigate the effect of temperature on the rate of reaction of the enzyme catalase. The biological catalyst called enzymes increase the rate of reaction without themselves being chemically changed. Enzymes are globular proteins folded into a complex 3-dimensional shape that contains a special surface area called the active site where a specific substrate binds structurally and chemically. Enzymes speed up reactions…

In this essay, I will explain how we can benefit from bonding of atoms of certain elements in our daily life. As I will show in this essay, with the help of understanding the surface chemistry, which is the study of chemical reactions at interfaces (surfaces), different desired affects can be produced by the formation of selected elements such as making improved chemical composition of a surface. Surface chemistry today can be applied to solve various issues and problems including fighting…

Nuclear magnetic resonance (NMR) spectroscopy and imaging can be used to investigate, noninvasively, a wide range of biological processes in systems as diverse as protein solutions, single cells, isolated perfused organs, and tissues in vivo. It is also possible to combine different NMR techniques enabling metabolic, anatomical, and physiological information to be obtained in the same experiment. This review provides a simple overview of the basic principles of NMR and outlines both the…

ABSTRACT Objective: To evaluate the in-vitro anti-inflammatory activity on leaves extract of Callistemon citrinus by using inhibition of albumin denaturation technique. Method- The different extract i.e. chloroformic, ethanolic and aqueous extract at different concentrations was incubated with egg albumin in controlled experimental condition and subjected to determine absorbance to evaluate the anti-inflammatory activity. Diclofenac sodium was used as the standard/ reference drug. Result: This…

Where Does Taurine Come From? Taurine (2-aminoethanesulfonic acid) is an is an organic compound discovered in bull bile in 1827 and mainly found in different animal tissues mainly in bile and large intestines. The term Taurine has a Latin origin from the word taurus which means a bull. Taurine has many functions inside animal bodies like muscle development, cardiovascular functions, and nervous system development among other functions. Once extracted, this acid compound is used in several…