Immobilized enzyme

A. Description of the Study Area The experimentation of the study will be conducted in PLM College of Science Laboratory at Pamantasan ng Lungsod ng Maynila, Gusaling Lacson, 6th floor. The University is located beside Department of Labor and Employment building in Intramuros, Manila, known as the “Walled City”. At the back of the University is the Philippine Red Cross - Manila Chapter. The Lacson Building where the laboratory is located is in between the University Gymnasium and Justo Albert…

this series of experiments the productivity and function of the enzyme Alkaline Phosphatase will be examined. Alkaline Phosphatase is an enzyme commonly produced and found throughout the entire human body. Its main function is the cleaving of phosphate groups from mostly organic molecules, including proteins, nucleotides and even alkaloids, which makes it a hydrolase enzyme (Ellis, 2016). In biochemistry, a hydrolase enzyme is an enzyme that speeds up the hydrolysis of complex biomolecules like…

salicylate hydroxylase (11), anthranilate hydroxylase (12), melilolate hydroxylase (13). Generally, the catalytic cycles of these enzymes follow the reaction shown in Scheme 3. The reaction is composed of two parts: the reductive half-reaction and the oxidative half-reaction. Scheme 3 shows the catalytic cycle of p-hydroxybenzoate hydroxylase, the most extensively studied enzymes of the class, and also represents a general model of reaction mechanism for other single-component…

5.3. Results of the 3rd experiment. (Co-digestion of kitchen waste with cow dung slurry). 5.3.1. Results for the physicochemical characteristics Sl. No Parameter Fresh cow dung slurry Fresh food waste slurry Fresh cow-dung + fresh food waste slurry Digested slurry 1 pH 6.4 6.0 6.2 7.0 2 Total solids (%) 8.5 9.1 8.8 6.16 3 Moisture Content (%) 92 90.5 91.25 94.33 4 Volatile Solids (%) 92.9 94.5 93.7 82.50 5 Percent carbon 2.27 3.03 2.65 0.27 6 Phosphate(mg/100g) 0.24 0.310 0.275 1.010…

NOSIPHO ZUMA A00018164 BIO 120 DR HAYATU RAJI LAB 1: PH TEST FOR VARIOUS SUBSTANCES LAB PARTNERS: AMINA SULEIMAN, SEDOO ORJE-ISHEGH DATE: 27 SEPTEMBER 2016 Abstract The primary purpose of this experiment was to determine pH levels of various substances using different methods. Two techniques were used, the pH paper indicator and the pH meter. Out of ten substances that were used, five of them were found to be acidic, namely: hydrochloric acid, coke, toothpaste, milk, and acetic acid. Sodium…

to determine reducing sugar content of food sample by performing Fehling method and to identify invert sugar content of sugars by using polarimetry and also to learn property of invert and reducing sugar. Carbohydrases represent a large class of enzymes hydrolyzing polysaccharides and low-molecular-weight glycosides. They belong to the class of hydrolases. Carbohydrases are classified according to their specificity…

Methylation is a key biochemical process that is essential for the proper functioning of almost every system in your body. It occurs billions of times per second; it helps repair your DNA on a daily basis; it controls homocysteine (an unhealthy compound that can damage blood vessels); it allows the recycling of molecules necessary for detoxification; and it helps maintain mood and keep inflammation under control. To maintain the proper functioning of methylation you need an optimal level of…

Cecilia Wong Nature of the task and the research question: Two centimetre piece of potato is bathes into different salt solutions (0%, 5%, 10%, 15%, 25%, 35%) over a period of time, to determine the effect of different salt solutions on osmosis. To investigate the effect of increasing the concentration of sodium chloride (0%, 5%, 10%, 15%, 25%, 35%) on the process of osmosis, by calculating the mass change of the potato core (g, ±0.01g) at room temperate of lab (25.0˚C) Table 1: Raw data Table…

Research Question: To what extent does temperature have an affect on the enzymatic rate of reaction between starch and amylase? Background Information: Enzymes and the function of enzymes are extremely vital to the human body in regards to how quickly the body can break down the macronutrients of starch, lipids, and proteins. I chose to investigate the breaking down of a complex carbohydrate (starch) because due to their size and structure, starch is broken down more slowly than other…

For the first part, the qualitative polyphenol oxidase test (Table 1), we looked at 3 qualitative test tubes, where only test tube 2 turned orange since this tube had both the 40 mM dopa, the substrate and PPO enzyme extract. However, the test tube 3 did have a cloudy component because of the presence of PPO extract, but there was no color change. For a color change to occur, there needs to be a presence of both the dopa and PPO extract. Furthermore, we calculated the final concentrations of…