Hydrolysis

Cellular respiration is a very interesting topic that we are currently learning about in our biology class. What makes this particular topic so remarkable are all of the different elements that work together for cellular respiration to occur in human beings and animals. In the video, “ATP & Respiration: Crash Course Biology #& 7.” (ATP & Respiration: Crash Course Biology #7. n.d.). The narrator does an excellent job of explaining the cellular respiration process in a very clear cut…

FLCN and RagB seen under starved conditions to its dissociation upon glutamine stimulation. Finally, I will observe FLCN-FNIP RagC/D GAP activity, whereby FLCN complex stimulates GTP hydrolysis on RagC/D when supplied with amino acids. FLCN-FNIP GAP activity will be tracked by thin-layer chromatography (TLC) GTP hydrolysis in protein mixtures of purified FLAG-RagC/D and FLCN-FNIP2 from cell lysates that were either starved or starved then re-stimulated with glutamine, which are then mixed with…

The focus of this experiment will be chymotrypsin which falls under the category of serine proteases. Chymotrypsin has been discovered as a digestive enzyme found in pancreatic secretions of animals, whose main function is said to be hydrolysis of peptides that enter the duodenum of the small intestine. As can be determined from its enzyme class, it has an active serine residue which is used to hydrolyze aromatic amino acids such as Phenylalanine, Tyrosine or Tryptophan at their C-terminus…

Gram Negative Unknown Lab Report #20 BIOL 3444-008 Giang Nguyen TA: Malini Kotak 11/05/2014 Abstract The experiment is conducted in order to identify a randomly given unknown gram-negative microorganism. By comparative analysis of biochemical tests that were performed throughout the semester to find out its characteristics and determine the identification of bacterium out of four potential unknown was given. To figure out the characteristics, T-streak was performed to isolate…

of enzymes: 1). Efficiency: the efficiency of the enzyme catalyst is higher than inorganic catalyst, therefore speed up reaction rates; 2.) Specificity: an enzyme can catalyze one or a class of substrates, such as proteases can only catalyze the hydrolysis of proteins into peptides. 3. )Diversity: there are many types of enzymes have been found , the number of enzymes in organisms is much larger than we discovered. 4. )Mild: It refers to a chemical reaction is catalyzed by an enzyme under…

60 minutes, however the shorter time period did not permit the maximum water absorbance to be reached (i.e. a plateau of the graph). Therefore, the course of this experiment was carried out over 160 minutes, to observe the decrease in the rate of hydrolysis. Different concentrated NaCl solutions were used as the solutions that the chickpeas were soaked in, altering the maximum amount of water absorbed. As shown in Figure 1, the more dilute the NaCl solution is, the more water absorbance was…

1. Introduction and background The experiment that will be conducted is the preparation of benzoic acid. Benzoic acid is a carboxylic acid and this has been established in the 16th century. The molecular structure of benzoic acid can be seen in the following figure. Figure 1 – Molecular Structure Benzoic acid is used to prepare esters with the very high boiling points and is used as plasticisers. Plasticisers are compounds that are added to plastics to…

and Function Lab, 03, 12:30 PM Instructor: Melanie Gustafso-Ropski – Lab Assistant: Corinna Monday March 9,2015 1. Part A • The enzyme used in this experiment is pancreatic lipase. The substrate for pancreatic lipase is lipids or triglycerides. Hydrolysis of triglycerides occurs in the duodenum and the PH rages are from 7.1 to 8.2. • Phenol red was used as a PH indicator of the samples. if the sample is an acid then a yellow color is appeared, if the sample is alkaline then a red color will…

Cassava starch can be changed over promptly to ethyl liquor in a two stage prepare including the hydrolysis of starch slurry into glucose by liquefaction so that dextrin and along these lines fermentable sugar can be acquired. The glucose arrangement is weakened and changed over to ethyl liquor by the anaerobic activity of yeast, ethanol of 95.6% w/w turns out through parchedness which is concentrated to 99.5% w/w (Ramasamy and Paramasamy, 2001; Kosugi et al., 2009). In this manner cassava-based…

defining the genus and species. All the following procedures were carried out on the gram positive cocci unknown in this order: Catalase, Oxidase, Nitrate Reduction, Oxidation-Fermentation, Phenol Red, Bacitracin, Coagulase, and lastly, Gelatin Hydrolysis. These tests led me to believe that unknown number 93 is Kocuria rosea. Discussion Looking at the dichotomous key, the first test that helps narrow down the bacteria…