measure the enzyme activity in each sample fraction. P-nitrophenol shows a linear relationship with acid phosphatase. An Enzyme assay gives the activity levels of acid phosphatase in each fraction. The results from each fraction were placed into the equation of the line from the standard curve of p-nitrophenol. A standard curve of p-nitrophenol is used because this is the product created after acid phosphatase is reacted with the specific buffer. The activity in each homogenate changed the…
RESULTS Phytic Acid The concentration of phytic acid was determined spectrophotometrically in three tested plant materials of Gishta as shown in table 2. The concentrations of phytic acid were obtained from 5 g dried leafy material. All the leaf samples contained phytic acid (detection limit 0.025 mg/ml) in this study. This may have been due to the fact that there was a limitation in terms of the detection concentration since concentrations less than mg was not tested. Seed extracts was found…
Sterile Ointments Ointments are semisolids expected for external application to the skin or mucous layers. They typically contain lower than 20% water and volatiles and more than 50% hydrocarbons, waxes, or polyols as the vehicle. Ointment bases known for use as vehicles fall into four general classes: hydrocarbon bases, absorption bases, water-removable bases, and water-soluble bases. Each therapeutic ointment has its base one of these four general classes. • Hydrocarbon Bases: They are…
RESULTS AND DISCUSSION The discussion will cover the obtained results under the following heads: 1. Effect of some different parameters on the behavior of drying rate Fig.1 showed the relation between drying rate and drying time under two drying methods of fruits peels. It was cleared that the drying rate decreased continuously with drying time. There was not any constant-rate drying period and all the drying operations are seen to occur in the falling rate period. The drying rates were higher…
The study of the “impact of artificial light on the production of sugars and starch by seedlings” was conducted to compare the effectiveness of seed artificial light compared to natural light to see if artificial light could be used as a substitute for artificial light. To produce the results to create a comparison, students conducted Starch Testing and Carbohydrate testing. The starch testing consisted 6 tubes, two of which were controlled samples with and without a Lugol’s Iodine, two…
Radioactivity past paper assignment Question 1: In the fridge, from a previous experiment, there is a 50 mM [U-14C] glucose solution. A 20 uL aliquot was counted and gave 200 000 dpm. What is the specific activity of this preparation? 20 uL = 200 000 dpm (divide both sides by 20) 1 uL = 10 000 dpm Glucose = 50 mM = 50 mmol / uL = 50 umol/ uL = 50 nmol / uL Therefore in one uL = 50 nmol/ uL = 10 000 dpm (divide both side by…
breaking down water molecules is called photolysis. In the dark reactions, glucose is made. Carbon dioxide and ribulose bisphosphate, which is also called RuBP, unite to form rubisco, which then splits to make two phosphoglyceric acid molecules, the phosphoglyceric acid is sometimes called PGA. PGA gets converted to phosphoglyceraldehyde or PGAL. Many PGAL is used to make more RuBP. This is called the Calvin Cycle. Photosynthesis is affected by many factors, like the amount of carbon dioxide in…
and many colors and glitters to use and many activators. My last science project that I did was which better juice cleans better pennies. The result was that orange juice cleans better pennies because orange juice has acid. Slime is easy to make because probably you will…
Sharpie permanent markers contain n-propanol (C3H8O) n-butanol (C4H10O), and Diacetone alcohol (C6H12O2). These markers are generally used on hard, non-porous surfaces, because instead of damaging the surface they form a surface layer that can be removed easily by solvents such as acetone (C3H6O), xylene (C8H10), or toluene (C7H8). Acetone (strong chemical used in nail polish remover) is a strong and very effective against permanent markers. It can also remove paint and melt certain surfaces.…
Project 3 Identification and Synthesis of an Unknown Ionic Compound Mitchell Morgan CEM-161-007 Josh Ward 28th October 2015 Abstract The chief objective of this experiment was to identify the unknown compound. The following tests were conducted to eventually establish the identity of the compounded: qualitative solubility tests, anion and cation tests, flame test, and the formation of a precipitate. The physical properties of the unknown ionic compound found that it has no smell and was…