High protein diet

subcortical infarcts and leukoencephalopathy (CADASIL) is the metabolic condition caused by a production of abnormal Notch 3 receptor proteins. The result of these abnormal proteins causes thickening of blood vessel walls that block the flow of blood to the brain. This is caused by a mutation in the Notch 3 gene. Cells containing abnormal Notch 3 receptor proteins results in disrupted normal functionality and ultimately leads to apoptosis of these cells. As the name of CADASIL suggests, it is an…

of small proteins. Keratin is the main component of your hair, skin, and nails. Integrin acts a sort of messenger. Cadherin acts a binder, creating the structure of cells. So many different proteins help make the world turn waiting the human body, but they do different things. These are three important proteins. Keratin to is very important to the human body. Keratin is a big factor in making up your hair, nails, and skin. In fact, your hair is 95% keratin This protein works…

The folding mechanism of knotted/slipknotted protein has become a challenging question over the last two decades.(10, 89, 90, 95, 99, 100, 173-175) Understanding how those proteins fold would be a great proceeding of the field of protein folding. Recent advances on protein folding shed light on this question using both experimental and computational methods. .(10, 89, 90, 95, 99, 100, 173-175) Most experimental studies on the folding of knotted protein have been limited to bulk experiments.(84,…

In Procedure 6.1, two different gels were used. One gel was 2.0% agarose while the other gel was 0.8% agarose. The reason we poured two gels was because each gel had a different purpose. The 2.0% agarose (thicker gel) was used to determine amplicons while the 0.8% agarose (thinner gel) was used for restriction fragments. One gel was ‘thicker’ than the other gel to distinguish smaller segments than the thinner gel. By having a thicker gel, smaller segments can move better and not be cleared out…

When Kevlar is spun, the resulting fiber has a tensile strength of about 3,620 MPa,[13] and a relative density of 1.44. The polymer owes its high strength to the many inter-chain bonds. These inter-molecular hydrogen bonds form between the carbonyl groups and NH centers. Additional strength is derived from aromatic stacking interactions between adjacent strands. These interactions have a greater influence on Kevlar than the van der Waals interactions and chain length that typically influence the…

phospholipids, cholesterol, proteins, and carbohydrates. The phospholipids and cholesterol both help create the roof of the cell membrane and they both regulate the fluidity of the cell membrane. The proteins in the cell membrane help provide a structural function, transport function, and signaling function all within the membrane (Yeagle, 1989). The carbohydrates are on the external side of the cell membrane. Also the carbohydrates provide a signaling function with the proteins and lipids. B.…

Caenorhabditis elegans C. elegans is a nematode worm that considers as one of the simplest multicellular model organism. It is a free-living soil animal that feeds on microbes in decaying plant environments while in vitro, it mainly feeds on bacterial lawn (40). Sydney Brenner has proposed C. elegans as an experimental model organism and it has been used in biological fields, it played a huge role in the understanding and development in the knowledge about multicellular organisms since 1960s (8)…

central dogma is the explanation of how genetic information flows as it is passed from DNA to RNA to protein. DNA replicates and converts the information it carries into RNA, this process is called transcription. During transcription the information is passed through nucleic acids and there is no need for a change in the “language.” When RNA continues the flow of information and it is passed to proteins, this is called translation. It is called translation because the information must be…

inhibition of cell motility. Larson and Satir (1991) suggest is could be because N2+ acts initially in the cell membrane and then directly on the axoneme (cilia structure). N2+ inhibits microtubule translocation by acting on 14S and 22S dynein ( motor proteins which activity cause bending movement). However, the research carried by Larson and Satir (1991) establish that the effect of Ni2+ is predominant on 14S dynein. This gradual effect on the inhibition of cilia in paramecium is also…

2.5.5 Western blotting Western blotting was done to separate and identify the size of proteins that react with a specific antibody. After the proteins was transferred onto the PVDF membrane, it was incubated in 5% blocking solution for 1 h at room temperature with constant swirling with belly dancer (Storall Life Science). The blocking of the membrane is to prevent the nonspecific binding of the detection antibodies during next step. After that, washing of membrane was done by using 0.05% TBST…