High protein diet

Report on Result and Discussion on isolation of protein from spinach leaves In this experiment, we tried isolating an actual protein called Rubisco from spinach leaves. The full meaning of these Rubisco means Ribulose-1, 5-bisphosphate carboxylase/oxygenase which is an enzyme that catalyzes the addition of a carbondioxide group to ribulose-1, 5-bisphosphate during sugar production in green plants. Although Rubisco is an abundant protein in plants, it is not the major component in all spinach…

1900s [1]. However, the proteins and enzymes involved in the process took a lot of time to identify its exact function and property. Ruford Zechner, a Biochemical professor at University of Graz, made a great contribution in the area of lipid catabolism and identified the properties of several important metabolic lipases involved in each step of the triglyceride hydrolysis into fatty acids. This led to the discovery of adipose-triglyceride lipase (ATGL) and its coactivator protein called CGI-58…

The muscle and connective tissue is found in the oesophagus. This is because the smooth muscle contracts which helps to move food along the. The cartilage is found in the esophagus is because it provides support and flexibility when the food moves. The oesophagus is where the food travels towards the stomach so that it can be broken down which then the body uses for energy. The nucleus controls the cell activities inside the cell. Mitochondria are needing to give energy to the cell and tissue.…

There are millions of genes that are present in the human body. For the most part, genes are responsible for pairing DNA bases with the correct base, and directing the production of RNA molecules, which synthesizes proteins and regulates the expression of other genes, but when some genes are altered or manipulated during DNA replication, they can be very dangerous for the body, and can be possibly deadly. Some of the genes in our body that are mutated can play a positive role in the body in ways…

Reaction) to amplify the COI (cytochrome Oxidase I) gene of a tissue. In PCR the DNA fragment is placed into a tube with DNA primers and Taq Polymerases. Taq polymerases are used because they are one of the few DNA enzymes capable of withstanding the high heat needed to separate the DNA fragment into two strands. After the strands have separated the temperature sis then called to allow the primers to anneal to the strands. The the the Taq polymerase extends the DNA fragment by adding…

chosen because of its relevance in my life as a musician due its application in marching snare drum heads. Kevlar's inception dates back to the late 1960s and early 70s in a DuPont facility, where a young woman named Stephanie Kwolek was researching high-performance fibers for use in reducing gasoline consumption in automobiles. Her team was to find a stronger material for reinforcing tires to allow them to be lighter, stiffer, and more efficient in gas consumption. One day during her work,…

A single Lactaid pill was grinded with a mortar and pestle. 120 mg of the ground up Lactaid pill was dissolved in 200 mL of 0.08 M phosphate buffer (7.7 pH) and the resulting solution was centrifuged to remove any capsule remains. Enzyme and Substrate Solution Preparation A blank tube was prepared by adding 5 mL 0.08 M phosphate buffer (7.7 pH) and 0.5 mL of 2.5 nM ONPG to a cuvette to zero the spectrophotometer with the spectrophotometer set to 420 nm. The substrate solutions were prepared…

Within the skeletal muscle cells, there are many organelles. Each cell contains a plasma membrane called the sarcolemma, covering the outer surface of the cell (Ivy Rose). The sarcolemma protects the muscle cell and allows for its shape to be kept. From the sarcolemma, tunnel-like extensions called transverse tubules pass through the muscle fiber (Ivy Rose). These transverse tubules connect the sarcoplasmic reticulum with the sarcolemma (Graham and Parker 117). The sarcoplasmic reticulum is a…

Mitochondrion is an organelle that exists within the cytoplasm of eukaryote cells, they are considered to be one of the most important organelles. Mitochondrion is usually oval in shape and can vary in size from 0.5 - 1µm. Mitochondrion is composed of a double membrane, the outer membrane is smooth and encompasses the organelle whereas the inner membrane is folded to form layered structures called cristae. In between the outer and inner membrane, is an area called the…

The proteome of M. paratuberculosis strain JTC303 CF was different from that of the CE. Specifically, CF proteins generally had lower pIs (4.4 to 5.5) and smaller masses (most s immune system loses control of the pathogen, or the pathogen begins active replication, the organism disseminates, and the host response shifts to a Th2 type immune reaction. The hallmark of this infection stage is production of detectable serum antibodies. A positive relationship has been shown between the level of…