Hemolysis

allows continuous growth and nourishment of bacterial samples that are streaked and grown on it. Blood agar is a differential agar plate medium that grows gram-positive cocci bacteria, and was used to make note of hemolytic patterns (α, β, or no hemolysis) of the particular gram-positive present in the sample. MacConkey agar is a selective/ differential agar media that is selective for gram-negative bacteria, and differential between coliform and paracolon bacteria through its use of the pH…

either be staphylococcus, enterococcus, or micrococcus. Using the gram stain, it can also be seen that the bacteria in the unknown was staphylo, which means the cells were grouped together. The identification of the bacteria was reached when the hemolysis test was done. “Hemolysin activity, when present, is usually weak”. (Breed) The test resulted in no lysis meaning…

incubated for 24 hours at 35 °C. In the third and fourth quadrants, two different bacterial cultures were present. The bacteria that covered most of the plate were light gray, circular, convex, entire colonies in a punctiform arrangement with slight hemolysis around the mixed sample in quadrant 1. The other bacteria were more sparse…

cells/hemoglobin contained in the blood stream.2 There is more than one type of anemia and they can be classified into Microcytic, Normocytic, and Macrocytic Anemias. Microcytic is usually caused by a deficit of iron in the blood, Normocytic could be due to hemolysis or acute blood loss, and Macrocytic is caused by B12 or Folate deficiency.1,2 Although these are the general causes of each type they are not the only causes for each type of anemia. Although there are many different types of anemia…

Question 3: Ethical Dilemma In clinical practice there are four principles that guide healthcare providers in bioethical decision making; these are autonomy, beneficence, nonmaleficence, and justice. The American College of Nurse-Midwifery integrates these principles into their code of ethics. According to the American College of Nurse-Midwives (ACNM) (2015), a midwife’s mission is to “promote the health and well-being of women and newborns within their families and communities”. In order to…

Paroxysmal cold hemoglobinuria is a disease that occurs in the cold. Paroxysmal cold hemoglobinuria, also known as PCH, mainly affects the hands and feet. In PCH, antibodies attaching themselves to the red blood cells. This gives other proteins in the blood the opportunity to attach as well. As the groups of attachments move throughout the body to warm themselves when in the cold scenarios, the antibodies destroy the red blood cells. As the cells are destroyed, the part of red blood cells that…

Diffusion is important because it is how particles are moved into a cell and out. Diffusion is the movement of a particle from an area of higher concentration to an area of lower concentration (Reasoner 2011). Passive diffusion and facilitated diffusion are the two major types of diffusions. Passive diffusion, also known as simple diffusion, is when particles move freely through the lipid bilayer of the plasma membrane (Tortora and Derrickson 2014). Facilitated diffusion is when and integral…

Sickle cell anemia (SCA) is a classification of the most common form of sickle cell disease. This class of sickle cell disease is genetic and is caused by the homozygous mutation of the beta-hemoglobin gene (beta S allele) in red blood cells [2]. Individuals who are diagnosed with sickle cell anemia have deformed (sickle shaped) red blood cells that have a lower lifespan [3]. This causes irreversible tissue damage and causes a vaso-occlusive crisis, which is pain in the abdomen, bones and chest…

Streptococcus is a genus of non-motile gram-positive cocci classified by serological types (Lancefield groups A through T), by hemolytic action (a, B, Y) when grown on blood agar, and by reaction to bacterial viruses (phage types 1 to 86). The various species occur in pairs, short chains, and chains. Some are facultative aerobes, and some are anaerobic. Some species also are hemolytic, and others are non-hemolytic. Many species cause disease in humans. Streptococcus faecalis, a…

and 0.01129 for triglycerides. The researchers added 1/5 and 1/10 dilution of the specimen and showed no changes in the TC value. They also used the icteric index to identify the patient’s total amount of bilirubin. Also, lipemic index and the hemolysis index were used during the procedure and showed no interference. The method that they used for measuring the patient’s sodium, potassium, and chloride was direct ion-selective electrode or also known as direct ISE. In fact, this analyzer…