Hemolysis

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    Red Pellet Lab Report

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    After being incubated in a water bath at 37 degrees Celsius for 20 minutes, the 8 samples were centrifuged at 400 rpm for 5 minutes and collected for observations. Presence of a red pellet in the first 7 tubes, including control Test Tube 1 were noted, while the red pellet was absent in control Test tube 8. It must also be noted that the pellet became progressively smaller as each successive tube was examined, a result more noticeable after examination of Test tube 5, as seen in Figure 1. The transparency of the solution changed in tandem with the colour of the solution. While Test tube 1 returned a clear and colorless solution, as successive tubes were examined the samples became more translucent and a red tint appeared by Test tube 4, growing more bold in colour with progressive Test tubes. This resulted in the final sample (Test tube 8) being a red translucent solution lacking the red pellet as shown in Figure 1. The incubator maintains a constant temperature of 37 degree Celsius to mimic the internal environment of the human body, which furnished the homes of various pathogens common to the human body. The red blood cells are incubated with the antibody under these favorable conditions so that the antibodies can initiate the cleavage of the C5 protein, allowing the membrane attack complex to form a pore on the sheep erythrocyte surface. Upon formation of this attack complex the cell present in the sample will begin to lyse, the amount of cell lysis dependent on the…

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    Footstrike Hemolysis Footstrike hemolysis happens when red blood cells in the foot break down faster than usual. This is usually due to repeated contact between the foot and the ground. This condition often happens to long-distance runners. It can also happen to other athletes, including dancers and hikers. This condition may also be called march hemoglobinuria. This condition may cause part of the red blood cells (hemoglobin) to appear in the urine, making the urine red (hematuria). In most…

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    Hemoglobinuria Essay

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    Patients with PNH often present with hemoglobinuria, abdominal pain, fever, headache and fatigue. While hepatic and cerebral veins are the most common sites of thrombosis, PVT is extremely rare with only about 12 cases reported in literature. Sparing of hepatic vein with involvement of portal vein and mesenteric veins makes this case highly unusual. A gastroenterologist must be keenly aware and consider the diagnosis of PNH in any young patient presenting with abdominal pain and hemolysis with…

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    Leukopenia Case Study

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    consistent with severe anemia including of pallor, fatigue, tachycardia, tachypnea, and hypotension. The patient’s new systolic ejection murmur is most likely related to increased semilunar flow due to the anemia. The recent throat infection and subsequent antibiotic treatment are the most probable cause of these symptoms. The patient’s leukopenia is also most likely a transient response to his infection, with another possibility being a drug reaction to treatment that he received for his…

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    From the second part of the example, cell burst is a possible situation can occur to the cell. In this experiment, cell burst, more specifically hemolysis, red blood cell death, lead organelles to spread in the solution and block the absorbance of light in the colorimeter. By comparison of the measured osmolarity and hemolysis between the different solutions provided the data to understand, how a red blood cell react in different osmolarity to maintain…

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    Mannitol Salt Agar

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    coagulase positive; otherwise the microbe is coagulase negative. The catalase test differentiates between microbes than can produce the enzyme catalase or not. If a microbe has the catalase enzyme, when hydrogen peroxide is added, bubbles appear making it catalase positive. If no gas or bubbles are observed, the microbe is catalase negative. A differential agar used for this experiment was the Blood agar. This agar differentiates between microbes that can create exotoxins to lyse the red blood…

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    B-complex which is formed by pyruvate yeast extract encourages growth. The selectivity of the medium is influenced by Lithium chloride and 1% potassium Tellurite solution which inhibits the growth of other organisms beside staphylococci. Coagulase differentiation results from the addition of potassium Tellurite and egg yolk emulsion. Staphylococci that has lectithinase breaks down the egg yolk and causes hemolysis around the colonies. After an incubation time of 24 hours the colonies appeared…

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    Microbiology Lab Reports

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    Staphylococcus epidermidis, Enterococcus faecalis, and Moraxella catarrhalis. After a Gram stain and observation of purple spheres under the microscope, the organism was learned to be Gram positive, thus eliminating M. catarrhalis. An oxidase test confirmed that both S. epidermidis and E. faecalis were oxidase negative since the organisms were clear and did not oxidize. To distinguish the two, a final confirmatory bile esculin test was performed. After incubation, the unknown tested positive for…

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    complications characterize this disease: - RBCs trapping and precipitation inside blood vessels - Inadequate vasodilation - Painful vasoocclusion episodes - Intravascular RBC breakdown (hemolysis) and release of their content of hemoglobin, heme, and iron ions, which are all strong oxidants - Anemia due to hemolysis - Toxic oxidative reactions due to cell free-heme damaging the endothelial cells of the vascular lining - Reduced blood flow and oxygen delivery (ischemic injury) in tissues and…

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    Catalase Test Essay

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    organisms into pathogenic and non-pathogenic. This is done by the indication of lysis (break down) of the RBC (Red Blood Cells) contained within the media. Many species of Gram-positive cocci produce exotoxins known as hemolysins, this is what destroys red blood cells (RBCs) and hemoglobin. Blood agar is a very common differential media selected to determine the species of pathogenic Streptococcus based on their ability to hemolyze RBCs. There are three hemolysis classifications an organism can…

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