Gram staining

1.Introduction: Background/Aim. 1.1 Nature of the microorganism: Enterococcus faecalis was placed under group D streptococci because of similar lysis characters; Identified as Gram positive, non-motile, anaerobic fermentative, lactic acid bacteria; are commensal inhabitant of intestinal bacteria and opportunistic pathogens. They occur singly or in pairs or chains. 1.2 Economic importance: Enterococcus faecalis is also used as a probiotic for production of Mediterranean cheese and meat products…

Materials and Methods Materials used in experiment 1.1A: Nutrient agar plate Materials used in experiment 1.1B: Nutrient agar plate, Sterile cotton swab In experiment 1.1B a nutrient agar plate was opened and left in an arbitrary spot in the laboratory and left untouched for three hours, at the end of the lab it was collected and incubated at . In part B a swab/swab rinse method was preformed in order to inoculate the nutrient agar plate. A sterile cotton swab was taken out of its packaging, an…

In this experiment, three strains of tetracycline resistant E. Coli were tested. First, the bacteria were diluted via serial dilution in order to more accurately count and quantify the bacteria. To ensure that the three different strains of bacteria are not cross contaminated, label everything either Farm A, Farm B, or Farm C depending on what is being tested. There are six agar plates for each farm, three of the plates are tetracycline plates (which are labeled) and three are unlabeled. For…

Hankin performed an interesting experiment to test if the antibacterial ‘substance’ in Ganges and Jamuna was volatile in nature. He heated the Ganges and Jamuna water in an open and a hermatically sealed tube (Hankin, 1896 and Hankin 2011- this is the English version of the original French paper translated by gangagen.com). He found that the antibacterial activity was retained when the water was heated in the sealed tube, but was lost in the open tube. Although the exact temperature and the…

unknown. These test includes: Gram Stain, SIM, Sucrose, Citrate, MAC, and EMB. Each one of these test are different and they will give a positive or negative feedback which is how we will know which bacterium I have. A gram stain is one of the most use stain in microbiology. The purpose of a gram stain is to divide microorganisms into gram positive or gram negative. In this procedure you will use Crystal violent, Iodine, Acetone, and Safranin. You use different dyes because Gram negative…

“See if you can guess what I am now … I’m a zit. Get it?” - Bluto Background: The purpose of the antiseptic lab was to show the effectiveness of multiple antiseptics: hydrogen Peroxide, Purell hand sanitizer, Water and Iodine. The procedure of the lab for the first day involved our group acquiring a cotton swab and using the swab to gather the Propionibacterium acnes (P. acnes) bacteria from various spots around our face, we then grabbed a Petri dish filled only with a thin layer of agar. We…

Throughout the course of the semester we have learned how different variables in an environment can affect the growth, change, and behavior of an organism. Using this research as a guide we set out to design an experiment that would show a particular variable’s effect on brine shrimp eggs. After much debate we decided to use light wavelengths as the variable being tested. We wanted to see the difference in development in the brine shrimp eggs and the development after hatching when…

evaluated for their anti-microbial activity using the agar diffusion technique . The tested compounds were evaluated against, Gram +ve bacteria , Gram -ve bacteria, Yeast and Filamentous Fungus. Minimum inhibitory concentration (MIC) was made in vitro for compounds which showed antimicrobial activity. Compound 3b has broad spectrum activity against Gram +ve bacteria, Gram -ve bacteria, Yeast and Filamentous Fungus with MICs 150, 150, 75, 312, 625 and 625 µg/mL against (Bacillus subtilis…

3. I chose the plate with dilution factor 10-7 to count. This was the only plate that yielded an appropriate CFU, therefore, it was the sample with the highest countable colonies. The plate with the dilution of 10-6 yielded Too Many To Count (>300) while the plates with dilution 10-8 and 10-9 yielded Too Few To Count (<30). 4.If I were to do a total count on this sample, as opposed to a viable count, the total count would be higher because it accounts for all cells, not just visible and…

If different abiotic environmental variables are offered to an earthworm, will they prefer one over another? We planned and carried out investigations by planning the experiments the worm was going to do, and we carried out investigations by investigating and writing down what happened. One cross-cutting concept that was in the lab was patterns because 3 out of 4 experiments ended in the worm picking the same thing 3 times. The first hypothesis is that if worms are placed in an area with light…