Gram staining

After receiving unknown organism #14, many tests were performed to discover the identity. After running a variety of tests, my results led me to believe that my unknown organism was Klebsiella pneumoniae. K. pneumonia produced a negative-rod gram stain. My organism, K. pneumoniae, tested positive for the three main carbohydrates, glucose, lactose, and sucrose. After running tests on these carbohydrates, a pH indicator color change from red to yellow signified a positive result, while remaining…

To determine the effect of the proximity of the dam on fish distributions downstream, I will collect data at three locations along the Monongahela River, moving downstream from the dam (Site 1, Site 2, and Site 3 in Figure 1.A). I will repeat this two times on two different days of similar weather patterns for a total of $110 in anticipated costs. At each site, I will collect a variety of variables. For starters, I will collect an indication of water clarity using a secchi disk at each site for…

INTRODUCTION On the 9th our Biology class went on a trip to Sirens Rock Wellington to investigate the organisms living at the rocky shore and their relationships with each other our task was to display information on the relationships between two species we discovered at the Rocky Shore we then use this information to form a report and explain the species and their relationship with one another, but before this we need to understand the definition of zonation; zonation is the distribution of…

Cells are the basic units of life. Each cell has different functions and properties that make life diverse. In this experiment, we had to deal with infections in two people. In order to treat the infections, we had to conduct a Grams’ test to figure out what kind of infection the children had and then identify the antibiotic that would best treat the infection or if they were antibiotic-resistant bacteria. For this experiment, my hypothesis is that both culture’s A and B will not be…

to discover the unknown bacteria was a gram stain. A section of the unknown bacteria was taken and stained with crystal violet and safrinin so that the bacterium was visible under a microscope. The gram stain gave the opportunity to know whether the bacterium was gram negative or positive. This allowed me to narrow down my options knowing that my bacterium was gram negative, using the unknown identification key. After discovering whether my bacterium was gram…

#7 was gram stained and observed under the microscope, the color appeared more pink than purple, leading to the conclusion that unknown microbe #7 was gram negative. However, when completing the KOH lysis test, the microbe didn’t become sticky and remained tacky which is how a gram positive KOH test would result. It was decided to double check the KOH lysis test and the second test still yielded the same results as the first, gram positive. The conclusion of unknown microbe #7 being gram…

The gram stain appeared purple so I concluded that bacteria two was gram positive. When observing it under the microscope it appeared to be coccus, in other words circles. They also seemed to be arranged in clusters. I performed the same tests on bacteria two, that I had performed on bacteria…

cell. To begin, place a drop of methylene blue on a slide, then smear a toothpick amongst the inside of the cheek, lastly smear the toothpick onto the stained slide. Cheek cells just like starch cells are hard to be viewed without the addition of a staining chemical, in this case, the methylene blue will make the cheek cells have a slight blue tint so they are more easily observed. Place the slide on the stage of the microscope then view and center the cheek cells under the scanning-power…

Purpose To identify an unknown microorganism by performing a series of biochemical tests on a pure bacterial culture. Materials and Methods 1. One test that was used to discover the identity of the given bacterial culture was the carbohydrate utilization tests. This specific set of tests involved two broths, namely phenol red lactose and phenol red sucrose, and a Durham tube placed upside down in the broth tubes. These broths can be broken down by fermentation processes,…

this was complete, we allowed the petri dish to incubate at 37°C for two days to allow the formation of new colonies. We then checked for growth/color change. If no color change was observed, we knew that the unknown bacteria was a salt-tolerant Gram-positive…