Gram staining

Determining the cell shapes will help with the process of elimination organisms. Under microscopic examination for my unknown 16 broth, it appears to be a coccus shape and a gram positive. Gram staining can determine cell morphology, size, and arrangement. In a positive gram stain, a primary stain called crystal violet is used to stain the cell. For every new stain, mordant, decolorizing agent, and counterstain, it must be rinsed with distilled water. Then mordant is added…

After gram staining both the gram positive and gram-negative bacteria, the groupings were observed. For the unknown gram-positive bacteria, the cells were arranged in a cocci cluster, while in the gram-negative bacteria, the cells were arranged in a rod cluster. Both the gram negative and gram-positive bacteria exhibit a small yellow colony on the TSA plate. After 48 hours, the MSA plate was observed. Individual isolated colonies were found throughout the plate, with a yellowish color. The…

Table 1 shows the all the isolates that produced a zone of inhibition for the ESKAPE pathogen, Staphylococcus aureus. In total, twenty six isolates were tested against Staphylococcus aureus. However, only eight showed a zone of inhibition. The size of the zones varied for each microorganism. Table 2 shows the results of the biochemical and morphological tests. The test that were performed were Sim, FTM, oxidase, catalase, MSA, blood agar, Acid fast stain and endospore stain. The sim and…

That week we performed a differential staining to determine whether our unknown has a gram-negative envelope which stains red or gram positive envelope which stains blue. Gram-negative microorganisms stain red because of their inability to bind crystal violet and their ability to bind the safranin. Gram-positive microorganism, however, stains blue due to their ability to retain the primary stain, crystal violet. I determined my unknown sample to be a gram-negative microorganism. After viewing…

1. Summary of how I identified Bacillus cereus. a. First, I performed the gram stain test, I then went on to do the spore stain. b. The results I obtained from the gram stain were gram positive rods, I could tell they were positive by the purple color to them and the rod shape by the comparison provided in the lab manual (Wilkosz, Peterson). After I confirmed the results I performed the spore stain, which indicated that my sample was positive so it contained endospores. The sample should have…

the Gram stain divides bacteria into two groups- these bacteria are grouped according to their differential staining properties. One group is Gram positive bacteria and the other group is Gram negative bacteria. The Gram positive bacteria have cell walls with thick layer of protein-sugar and low content of lipids. During the decolorization…

Introduction: The name Pseudomonas is derived from the greek and latin words which means “false unit“ (pseudo = false, greek; monas = single unit, latin). “Monas” was used in the early history of microbiology to define single-celled organisms. In 1786 Otto Friedrich Müller, from Copenhagen, classified the bacteria and named the pseudomonads, they came into the group of vibriones (which was defined as group of shaking bacteria). Many years later it was detected that Pseudomonas are motile. They…

Following the Ways of the Scientific Method The scientists solved the Elkhorn Coral mystery using the scientific method by following each step of the method to come to a conclusion as to what is killing the coral. The followed the steps precisely so that would not make any errors during their trials. They started with the first step of the scientific method, making an observation. Paragraph 7, sentence 1 of “Underwater Whodunit: What’s Killing Florida’s Elkhorn Coral” states, “Hot on the trail…

In order to collect accurate data from an experiment, it is important to evaluate the environment that the lab will be taking pace. While my lab partners and I were doing this, we checked to see if the workspace is clean and clear of an unnecessary or harmful objects, especially food. Then our second step was to gather all the necessary supplies for the experiment. This included, a microscope, three glass microslides, alcohol, caffeine, and water, four pippets, then finally three water fleas.…

Introduction Termites, Isotera, are known to react to the exposure of the chemical substance found in ball point pens. Naturally, termites will follow any trail made up of pen ink. This specific observation was tested and carried out by Sim et al. They “investigated how termites select the direction of movement at an artificially-designed branching node, by making chemical trails on filter paper, drawing lines using a ballpoint pen which contained the chemical substance that induces the termite…