Gram staining

The second test tube 11 was identified as a gram negative bacteria. The biochemical tests that I performed that were positive were catalase, glucose, SIM, indole, citrate, and urea. First, the catalase test has the presence of an enzyme in the test isolated detected using hydrogen peroxide. Bacteria possess catalase with a small number of bacteria; isolation is added to hydrogen peroxide when bubbles occur. When the bubbles occurred on gram positive bacterium it was a conformation of being…

Serratia marcescens 1b. Glucose not fermented 8a. Glucose oxidized…………………………….........…………Pseudomonas aeruginosa 8b. Glucose not oxidized…………………………………………......Alcaligenes faecalis Test Question being asked Observations Interpretations Gram Statin Is the unknown gram…

This genus of bacteria is known to be aerobic, gram-positive, non-motile bacteria. With shapes within colonies varying from rods to cocci (Finnetry 1992). This general knowledge backs up the conclusion and identification of the bacteria cultured in this study. Rhodococci are known to be widely spread…

The Enzyme Electrophoretic Separation of Proteins lab experiment will take place on March 7th, 2017, from 2:00pm to 5:00pm. It is important for students to follow lab safety guidelines in order to prevent any complications that may be a risk to one's health. For this experiment, students must wear their lab coat, goggles, and gloves at all times. All materials in this experiment should be handled with caution to avoid any damages. Students must keep hands away from face, eyes, and mouth, when…

with the use of a staining procedure which allows for greater contrast between the mostly translucent cells and light used by the microscope. Staining is a critical step when analyzing microorganisms and has great significance in a laboratory or clinical setting. The two different staining…

shape, cell wall structure, and other characteristic using the results from tests such as gram staining, simple staining, and spore staining. According to my lab notebook, the result from gram staining of my known is seen to be a rounded shape, cocci shape all bunched and clustered together like a bunch of grapes at 100x using the microscope (Kaddo, 2017, pg. 22). The color after the safranin wash from gram staining…

The Gram stain is a valuable tool to microbiologists in identifying the cell well composition of prokaryotic species. The underlying process revolves around a series of chemical dyes, and their propensity to bind to peptidoglycan polymers in the cell wall. The results allow scientists to infer properties about the outer coating of an organism and is often the first step in identifying an unknown species. For the purpose of our experiment, the results of the Gram stain will be as follows: Gram…

Game positive or Gram negative, after Gram staining, there will be an ability to view the morphology or shape of this bacteria. The doctor can further test for the bacteria present, after the doctor uses gram staining, they can then use the catalase test, and polymerase chain reaction (PCR) tests to further positively identify the…

bacteria. Organism number thirteen is Escherichia coli. On the first day, I conducted a gram stain and noticed that the bacteria were pink, meaning that the bacteria are gram negative. I also recognized that the bacteria were rod shaped therefore they were bacillus. I also noticed that the arrangement appeared to be single celled. I also put the bacteria on a streak plate. On the second day, I did another gram stain and the bacteria were again pink and bacillus or rod shaped. I also observed…

inoculated with our unknown organism it was allowed to be incubated at 35 degrees Celsius for 24 hours. After the plates had undergone incubation for the full 24 hour duration, color and morphology was observed and noted for our unknown organism. Gram staining was than conducted after, in which we determine if the unknown organisms was either positive or negative based on the thickness of its peptidoglycan wall that was able to retain the crystal violet color or not. This determination lead to…