Gram staining

bacterial ribosome. This ultimately acts to block the exit of the growing peptide chain. It should be noted that gram+ bacteria more effectively accumulate erythromycin than gram negative bacteria thereby making it more effective in this group of bacteria. Erythromycin is used to treat syphilis, group b strep, respiratory tract and skin infections. Resistance to erythromycin can evolve when gram+ bacteria develop pumps which export the drug thereby protecting the bacteria from the drug’s…

Recommended Testing Lactate Level Blood Cultures X 2 (Different Sites) - Prior to initiating antibiotics Culture Surgical Site and Gram Stain Complete Blood Count with Differential (CBC) Complete Metabolic Profile (CMP) Blood Glucose Monitoring Prothrombin Time (PT) and International Nectrotizing Ratio (INR) Activated Partial Thromboplastin Time (aPTT) Urinalysis (UA) and Culture CT Abdomen/Pelvis Radiograph of Right Lower Extremity The given scenario leads this writer to pursue aggressive…

Stain 4. Prepared Agarose 0.8% 5. Tris-Borate-EDTA 5x Buffer 6. Electrophoresis Mold 7. Electrophoresis comb 8. Electrophoresis chamber 9. Electrophoresis power pack and cords 10. DNA from cow liver 11. DNA from chicken liver 12. 224 grams of fresh cow’s liver 13. 224 grams of fresh chicken liver 14. Meat tenderizer 15. Kitchen knife 16. Blender 17. 2.5 ml Salt 18. 31 ml dishwashing liquid (Dawn) 19. 354 ml warm water 20. Small test tube 21. Beaker 22. Cheesecloth 23. 354 cold isopropyl…

Laboratory Response Network). The nurse transported the sample to the laboratory at room temperature; about 70°F. Microbiologist in the laboratory performed series of test to identify the disease Molly has. One of which was gram staining on the specimen. The results from the specimen were gram- positive when observed under the microscope because it stained purple. Additionally, this revealed the specimen cell morphology is bacilli which are rod-shaped and the cell arrangement of the specimen was…

is to categorize it as Gram-positive or Gram-negative. Bacteria is made up of different chemical composition of cell walls, this allows for the gram test to classify between the two major types. Gram-positive bacteria has a thick layer of peptidoglycan located in the cell wall, while Gram-negative bacteria have a thin layer of peptidoglycan layer in the cell wall. The Gram test is a staining technique that can detect the peptidoglycan in the cell walls of bacteria. The Gram-positive bacteria…

of the bacteria known as Staphylococcal aureus (Bauman 2015). This gram-positive bacteria is part of the normal human flora, meaning this bacterium does not usually cause infection; this type of bacteria can be found in moist areas of the body such as: the groin area or the nose (Toxic Shock Syndrome (TSS)). The thick peptidoglycan layer helps the bacteria’s cell wall to absorb the purple color. The color is then retained after grams iodine is applied. The color remains in the bacterium’s cell…

Sydney Meyers Relative effectiveness of penicillin, neomycin and erythromycin on the inhibition of growth of Streptococcus pneumoniae Introduction: An approximate 1.1 million annual deaths worldwide are attributed to the to bacteria known as Streptococcus pneumoniae. Today, S. pneumoniae is among the most significant causes of bacterial disease in humans and remains to be one of the top 10 causes of death in the U.S (Hoskins, 2001). This pathogenic bacteria causes a variety of…

Introduction Clinical laboratories have relied historically on phenotypic methods (i.e. culture and biochemical tests) to identify microorganisms. This can be time consuming task especially with slow growing organisms such as Mycobacterium tuberculosis. Molecular methods are now well established in the diagnosis of infectious disease. . (Harwood 2012). For the purpose of this essay I will give a brief overview of both traditional and molecular methods that re used today. I will focus on the pros…

colour bath bomb, while it is fizzing as temporary colour transfer may occur. If colour transfer to the skin does occur, it will remove itself after a few minutes of laying in the bath, no soap, no scrubbing, no problem! We only use water soluble, non-staining cosmetic grade colourants.  Due to the handmade nature of this item, please allow for slight variations in color, design, size and weight. Each one is unique! If you have any questions or concerns, please contact me. The full…

The Extraordinary Science of Microbiology There are a wide variety of microorganisms in this world. The major groups of microorganisms include bacteria and fungi. These microorganisms are found all over the earth. The study of microorganisms started in the mid-1600’s. Most people believed that microorganisms only spread deadly diseases, but scientists proved that microorganisms can also be beneficial to human life. The wide variety of microorganisms that are found everywhere are most well…