Gram staining

Results: In order to identify the gram positive isolate in the unknown #260 broth, I observed the bacterium under the microscope, created a stock culture, and conducted a variety of biochemical tests. First, I prepared a gram stain of the mixed culture using aseptic technique, shown in Figure 1, and found two different bacteria. There were too numerous to count purple cocci in clusters, pairs, and some single cells, which I identified as the target isolate. The other bacteria present a pink…

Introduction The existence of Chlamydia trachomatis has only been acknowledged for a little bit over a century. The microorganism was discovered in 1907 by scientists Ludwig Halberstaedter and Stanislaus von Prowazek when they detected it in scrapings of the conjunctiva of an infected orangutan (Budai, 2007). The duo found that similar microbes were found in humans as well. The affected areas were the urethra of males, cervices of women and the eyes of their infants (Dimitrakov, 2002).…

Figure 3: Total magnification of 1000X of the Gram-positive S. aureus Antibiotic Penicillin Novobiocin Kanamycin Control Zones of Inhibition (cm) Sample 1 3.5 2.3 1.5 0 Sample 2 3.5 2.5 1.5 0 Sample 3 3.0 2.1 1.7 0 Sample 4 3.6 2.5 1.5 0 Average 3.4 2.3 1.6 0 Standard Deviation 0.271 0.163…

Oklahoma’s campus. The nose of this statue was swabbed and is where one’s environmental isolate originated. Through morphological, physiological, and biochemical experiments, one was able to gather results that revealed that the environmental isolate is a Gram-negative bacillus that grows best at 37 °C. Additionally, it is a non-motile obligate aerobe that produces endospores, possesses a capsule, and is a non-hemolytic microorganism. Through these experiments along with other significant tests…

Results: The data gathered displays that there were 51 colonies in quadrant A (the handle of the paper dispenser in the women’s bathroom), most were circular in shape, white in color, and smooth over all. There were 87 colonies in quadrant B (the spatula used in the sandwich bar), most being circular, white, and smooth. There were 13 colonies in quadrant C (the outer handle of the front entrance to the school); they were mostly circular, beige and shiny. Lastly, there were 55 colonies in…

erythema migrans rash (bull’s-eye rash). First-line treatment is oral doxycycline, amoxicillin, or cefuroxime, which have equivalent efficacy for the treatment of Lyme disease. Doxycycline is not recommended for children under the age of 8 because tooth staining may occur. It…

Results: In order to identify the gram negative isolate in the unknown #260 broth, I observed the bacterium under the microscope, created a stock culture, and conducted a variety of biochemical tests. First, I inoculated a blood agar plate and a MacConkey (MAC) agar plate with the unknown #260 mixed culture broth. The blood agar plate, shown in Figure 1, was inoculated using the quadrant streaking method and then incubated for 24 hours at 35 °C. In the third and fourth quadrants, two different…

washed my hands and sanitized the room and sterilized the equipment, but that sneaky gram positive, coccus shaped bacterium just was able to grab onto my skin. I go to the gym and USE to place my phone between my pant and hip and well when you run you sweat so the bacteria ran into a hair follicle and well a folliculitis formed. Easy as that, 24 hours later I was sitting in the doctor’s office getting a cefazolin 1 gram deep intramuscular injection in my buttock and placed on an oral antibiotic…

Following Gram staining and microscopic examination, Unknown 15 was identified to be a Gram positive coccoid. Based on these results, all of the Gram negative genera were eliminated including: Enterobacter, Citrobacter, Klebsiella, Escherichia, Pseudomonas, Serratia, Alcaligenes, Neisseria, Proteus, Salmonella, Shigella, Erwinia, Veillonella, and Flavobacterium (5). Bacillus, Lactobacillus, Listeria, Kurthia, and Arthrobacter were eliminated because they did not match the coccoid shape of…

Yersinia pestis One of the five bacterium I identified was Yersinia pestis. It is a gram negative rod and these are the tests that identified it (and the results of those tests): gram morphology (g-rod), oxidase (negative), lactose fermentation (negative), indole (negative), urease (negative), motility (negative), orthine dicarboxylase (negative). These tests were chosen as directed by my flow chart. Discovery During a plague epidemic in Hong Kong in 1894, Yersinia pestis was discovered by…