Gram staining

that oyster. There is a few reports that describe the bacterial diversity which is poorly know and required to be further study.(Hernández and Olmos,2006). So, it is important to analyze the bacteria of oyster. This study is carry out to analyze the gram positive bacteria diversity present oyster, Crassostrea iredalei by applying molecular technique like DNA extraction and using of polymerase chain reaction (PCR). This study permit us to identify what and how bacteria that become pathogenic to…

organisms. They carry or produce toxins, which act as antigens that can be identified as foreign by the host. The toxins pathogens produce are of two general types; exotoxins and endotoxins. Generally, endotoxins are produced by gram negative bacteria and exotoxins are produced by gram positive bacteria (Conjecture Corporation, 2016). The few pathogenic species of bacteria are highly infectious have they have effective modes of transmission. Pathogens can be spread many ways, such as by coming…

two may not be significant (Figure 2). These results support part of the hypothesis as the two species of bacteria developed different zones of inhibition when exposed to the three antibiotics. Gram-staining confirmed the identity of S. epidermidis as a gram-positive bacteria and S. enteritidis as a gram-negative bacteria.…

most active against S.aureus. The assumption that Staphylococcus aureus would be more susceptible to the bactericidal effects of the tested antibacterial agents than Escherichia coli was based on the species’ classification as gram-positive. In order to be classified as gram-positive, a…

their choice. Unknown test tube #25 was obtained and streaked onto two agar plates to obtain individually isolated colonies. The gram-staining procedure was used to determine organismal morphology, resulting in a gram-positive cocci and gram-negative bacilli. A sucrose utilization test was performed and narrowed the possibilities of the gram-negative…

Neisseria meningitidis is the leading cause of bacterial meningitis in children and young adults. Extrameningeal manifestations of Neisseria meningitidis infection are uncommon in normal children and are a consequence of invasion of the bacterium into the blood stream. We describe a case of IMD with meningoencephalitis, septic arthritis, myositis and pleural effusion who developed secondary fever and prolonged hospitalization. Case report: A 10-year-old boy previously healthy was referred from…

anthracis, Yersinia pestis and others show anti-capsular antibodies in their serum. Capsule is impermeable to biological dyes routinely used, therefore they cannot be stained. Negative staining which colours the background is employed to demonstrate the capsule. India ink and Nigerosin are the two stains used for staining the background and later a simple stain like safranin or crystal violet is applied to stain the…

Yersinia petis, which better known as the plague, is caused by a gram-negative and rod-shaped coccobacillus bacteria. (Gram-negative meaning the bacteria does not contain the crystal violet stain involved in the gram staining test or process). The bacterium is also considered to be partially anaerobic, meaning it can function with oxygen but can also switch to a fermentation without oxygen. Yersinia petis function and survive through the lives and cycle of rodents and fleas, because of this,…

presence of a host cell, and it do not form spore. What special about this type of bacteria is that they are strongly acid fast bacteria, they are not gram negative and not…

Tryptic Soy Agar (TSA), the Colistin-Nalidixic Acid agar (CNA), and the MacConkey plate (MAC). Each plate had growth of coccus-shaped colonies. Through Gram staining, it was observed that the colonies on the TSA plates contained both gram-negative bacteria and gram-positive bacteria. Gram-positive bacteria were present on the CNA plate, and only gram-negative on the MAC plate. An observation was noted on the MAC plate, pink precipitation was observed on the bacteria colonies indicating that the…