Gram staining

plate showed growth of two different colonies. After the morphology was observed and recorded, a Gram stain was performed on each of the two unknowns, this procedure is found in the laboratory manual. The two microorganisms were identified; one was a Gram-positive rod bacteria, the other was a Gram-negative rod bacteria. A series of biochemical tests were performed based on the results of the Gram stains. The proper procedure…

MATERIALS AND METHODS The first day of the independent experiment was on October 25, 2016. Mannitol Salt Agar and Phenylethanol Agar are selective media that were used to isolate M. luteus from the environmental samples. Sterile cotton swabs were used to obtain the samples from the skin, arms and dust through wiping the surface of the sample source. A handful of backyard soil was previously diluted and then 2mL of this diluted soil was transferred to a sterile test tube containing 9mL water…

(Move to treatment??) Researchers have discovered that the fight against invading B. anthracis begins with the first infected cell. They found that the impacted macrophages will instantly communicate with other immune cells to warn them of the distress and develop a strategy in order to survive. The key signaling molecule that is involved in the survival strategy is adenosine triphosphate or ATP, which is a basic currency of energy transfer used by all organisms for various biochemical processes…

process allowed the unknown bacteria to grow and maintain. Before a series of test were conducted, first a gram stain test was performed in order to determine the unknown’s gram reaction and its cell morphology. After the gram stain procedure the unknown bacteria appeared purple and roughly spherical, when seen through the microscope. Thus, this concluded that the unknown bacterium was gram positive and its cell morphology was cocci. After this, a series of test were conducted in order to…

Gram + Cocci To identify the Gram + cocci I first performed a catalyst test which produced oxygen bubbles and indicated a positive catalase result. The next test I preformed was a mannitol salt test using a mannitol-salt agar plate and the results showed positive fermentation turning the plate yellow and also positive growth. This led me to conclusion that my unknown Gram + cocci was Staphylococcus aureus. Gram – Rod To identify the unknown Gram – rod I first performed a H2S production test…

routine culture; gram stain, and susceptibility testing requested. The methods and procedures that was learned and followed in this report are written in…

Introduction: Klebsiella pneumoniae is a part of the family Enterobacteriaceae. This genus is a gram negative and it has no flagella, which meant that this bacterium is not motile. However, it can move with the use of the current that is produced by the water. This bacterium has the ability to cause destructive change in a man or animals lungs if aspirated. In addition, this is also one of the most contacted pathogen in a Hospital Acquired Infection (HAI) or nosocomial infection. In this…

Introduction: Microbiology is the study of all living microorganisms like bacteria, fungi, protozoans…etc, and non-living microorganisms like viruses, viroids and prions. The significance of identifying an unknown microorganism is first to understand what type of organism it is and what structures it has to help figure out their potential benefit, danger or even neutrality to humans. Physicians benefit from this by identifying what their patients are suffering from and how to treat them. Also…

Three agar slants and three nutrient broths were inoculated with Staphylococcus epidermidis using aseptic techniques for transferring samples from broth, agar, and plate cultures. A sterile inoculating loop was used to transfer a loopful of culture from the culture to the sterile media. The lip of the culture tube and inoculated media tube was flamed before and after transfer. The inoculating loop was reserialized after transfer was complete. An agar deep transfer of S. epidermidis was also done…

Van Tran Microbiology Tuesday 5:00 May 5, 2015 Unknown Sample # 7 1. Introduction: Using biochemical tests are a good way to identify unknown bacteria from a sample. It is basically a similar to a trial and error method, but rather than using trial and error a person would be using positive and negative results. Using biochemical tests will help a person identify certain characteristics of the unknown bacteria through a series of chemical tests. Of course more than one biochemical tests…