Gram staining

from a gram positive and the other to be gram negative bacteria. One of which will be a cocci and the other a rod shape. The most important part of the study of bacteria is the knowledge of its identity. With the right approach knowing a bacteria’s identity is not as hard at all. Just as long as someone knows what he or she is doing these will go smoothly. First, we have to grow them in differential medium for culture separation. Then after 48 hours of incubation we started to identify the gram…

and ultrasound before aspiration. There may be an obvious deformity or cause of the bursitis, but if radiographs come back normal then aspiration can be performed. Of the many tests on bursa fluid aspirate, Gram staining is one of the most precise (Baumbach et al., 2014). Although Gram staining is so accurate, it was used less than 50% of the time by physicians and orthopedic surgeons in Switzerland (Baumbach et al., 2013). Each type of bursitis, SB or NSB, can be treated conservatively or…

Isolated Colonies and Soil Samples A. Purification of Serratia marcescens and Staphylococcus epidermis The objective was to grow isolated colonies on a nutrient agar medium (NA). Many microorganisms found in soil are able to grow on this medium. Since there are many organisms that are found in the soil, only a few of this organisms are adapted to live in this specific medium obtained. The NA plate is the first way to isolate microorganisms because the organisms that will be left on the plate…

Hypothesis: That Domestos is more effective in killing both the Escherichia coli and M bacteria than Dettol Pine o Clean and White Vinegar. Pine O Clean is more effective on gram-positive bacteria than gram negative bacteria, whereas Domestos has the ability to effectively destroy both gram negative and positive bacteria. Safety precautions: This experiment uses Pine O Clean Dettol, the person conducting this experiment should be cautious not to let the disinfectant come in contact…

Microbes of the infected root canal system Historically, the most wide-spread method for detecting and characterizing bacteria has been by culturing. Culturing bacteria allows the analysis of their morphology, virulence, gram-staining pattern, the quantization of viable microorganisms, as well as the testing of their susceptibility to antibiotics. Consequently, this method may not allow normally viable bacteria to cultivate as the selected culturing media may not have the nutritional and…

appearance of the bacteria’s, a gram stain was conducted on the isolated bacterial sample. The stain process is extremely essential because it separates the bacteria in two groups of gram positive and negatives bacteria’s .in addition, the gram stain shows the color, shape, size, and morphology of the bacteria. In order to narrow our search for unknown microbe, acid fast, spore and capsule stain were…

first thing to do is streak the unknown samples to obtain individual colonies for additional analysis by using a sterile loop to streak the bacterial samples into TSA for incubation. Next is the Gram Staining step to determine the morphology and Gram of the species using Crystal Violet for 1 minute, Gram iodine for 1 minute, 95% ethyl alcohol for 10-30 seconds, and Safranin for 1 minute, and then when you take a sample, examine it under a microscope. Next use MSA and Blood Agar. Use a loop to…

within the cells. (Staining. (2017, February 28). In Wikipedia, The Free Encyclopedia. Retrieved 11:28, April 11, 2017, from https://en.wikipedia.org/w/index.php?title=Staining&oldid=767802899) Cell staining is used to better visualize cells and its components such as nucleus and cell wall under a microscope which would otherwise not be seen clearly. Stains can be used both on living and non-living or fixed cells. Enumeration of cells to determine biomass is done through cell staining (Bruckner,…

The Mixed Broth culture contained two unknown bacterial cells and they either Gram-negative or Gram-positive. The procedures were followed as stated from the course laboratory manual by Meramec Community College (1). The first step was figuring out the unknowns was to separate the two bacteria. In order to do this, the Trypticase…

S, 2004). Their anatomy features Gram-positive, nonsporulating, polyploidic cocci, lacking flagella and motility and resistance to hydrogen peroxide and other agents that damage DNA because of a highly efficient DNA repair system. Although they are considered Gram-positive, they may be an intermediate between gram-positive and gram-negative bacteria and form a distinct bacterial lineage of extremophiles; the Firmicutes phylum has…