Gram staining

of the unknown specimen. This study was done by applying the methods learned in Professor Sprenkles microbiology lab in determining the unknown bacterial growth present with use of pure culture colony morphology, cell morphology and arrangement, and Gram reaction. MATERIALS AND METHODS The methods that have been taught for identifying a bacteria have been applied to determine the unknown bacterial growth. The first procedure performed was obtaining a culture from the NA plate, Section 2…

frequently trying to culture and classify microorganisms in a laboratory setting for study. Because of this there are now numerous test that can be used to identify unknown microbes. In this experiment, morphological characteristics, biochemical and gram staining test were used to identify unknown 5. These tests are important because most microbes are comparable…

Abstract This paper talks about a clinical case study that describes the condition of a 60 year old man. I have identified that this patient presents symptoms of peritonitis. Peritonitis is the inflammation of the peritoneum and is usually caused by diverticulitis which this patient has, also the patient has a cloudy peritoneal fluid which indicates the sign of a bacterium called Bacteroides fragilis. This bacterium is obligately anaerobic and is typically found in the digestive tract of humans.…

It is not a differential medium, but it is selective because Gram-negative organisms are inhibited by the phenylethly alcohol. Nutrient Agar was used to compare the growth quality of the organism to other mediums. Mannitol Salt Agar was another medium that was spot inoculated with the unknown. Mannitol Salt Agar…

Disease: Strep throat Causative Agent: Streptococcus pyogenes Morphology of Causing Agent: The bacterium S. pyogenes is spherical and emerges in chain-like structures. Gram-staining reveals that this bacterium is gram positive since it shows up dark purple. Transmission: Strep throat is highly contagious and primarily spreads when airborne by coughing or sneezing. Diagnosis: The most common way to diagnose strep throat is by having a trusted doctor perform a rapid antigen test after collecting…

for a regular bacteria cell meaning that it is bacteria. [Plus, it’s got it in its name ;)] 2.) Salmonella-I knew this was Salmonella because it was rod-shaped gram-negative bacteria. Gram-negative bacteria do not retain the crystal violet dye used in the gram staining method due to the fact that they have cell walls that are thin. Gram-negative bacteria are often harmful to a host, which is the case for many of the Salmonella bacteria. Protists 1.) Irish Moss- I knew that this was Irish Moss…

Additionally, under a microscope, each bacterium tested either positive or negative with a gram stain test. As shown in Table 1, of Experiment A, the Staphylococcus Aureus consisted of a perfect circle shape, it had tested positive for the gram-stain test which meant that the stain of the bacterial cells was purple, and the peptidoglycan cell walls were thick. Furthermore, Pseudomonas Aeruginosa tested gram negative which places it with a thin peptidoglycan cell wall. This type of bacteria…

difference in characteristics between Gram-positive and Gram negative bacteria, where one of most distinctive differences is that Gram-positive bacteria composes of a thick layer of peptidoglycan enveloping the plasma membrane, where as Gram-negative bacteria only have either a very thin or virtually do not exhibit the peptidoglycan layer. This trait of the Gram-positive bacteria causes them to be stained as purple color when washed with alcohol and water during Gram staining as the crystal…

Regan Hansen – A006569939 Biology 3300 - 503 Library Unknown – Neisseria Gonorrhoeae The characteristics that I had to look for was a gram negative coccus, often in flattened pairs, non-motile, aerobic, acid from glucose but not maltose, nitrites not reduced, and isolated from a sick human. I found out that there seems to be a very small amount of gram negative coccus bacteria. The biggest factor that helped me in determining my unknown was seeing that the acid came from glucose and not…

1. The biggest potential source of contamination of the locations we tested in the Distribution of Microorganisms would have to be the floor of the lab. The reason why this is the biggest potential source of contamination is because multiple people are coming in everyday from the outside world, bringing in microorganisms from the outside into the lab. And although we sanitize the counter and all the spaces that we work around, we don’t take precautions to necessarily clean the floor, making it…