Gram staining

The endospore is a quiescent and tough structure that is form by some gram-positive bacteria that allows them to survive in harsh and extreme environments. Because only some genera of bacteria produces the endospore, its identification is important in helping us identify some bacteria. 2. What type of eukaryotic cells did we observe and how were they different from the prokaryotes we have looked at? (Be sure to include ways they are different internally as well as any observable traits) The…

What is the ideal staining for identifying a Gram positive bacteria – Please explain your answer? The ideal staining for identifying a Gram positive bacteria is brown-brenn gram stain is preferred to detect the positive bacteria. 13. What is the ideal staining for identifying a Gram negative bacteria – Please explain your answer? The ideal staining for identifying a Gram negative bacteria is the brown-brenn modification is superior for the detection of the gram negative bacteria. 14. How…

The slide was washed, blot, and examine under microspore. C. ACID FAST STAINING: The lipoid capsule of the acid – fast organism takes up carbolfuchsim and resists decolorizatin with a dilute acid rinse. The lipoid capsule of the mycobacterium is of such high molecular weight that it is waxy at room temperature and successful penetration by the aqueous based staining solutions (such as Gram’s) is prevented. PROCEDURE: Smear of organisms to be stained were prepared…

Bacteria also known as prokaryotes are very ancient single-celled organisms; fossils show that they were widespread 1.5 billion years ago. Today bacteria make up the vast majority of prokaryotes on earth due to the unfavorable ability to adapt quickly to the surrounding environment. Bacteria have the capabilities to survive in areas of high heat, extreme cold, or even very acidic or alkaline conditions. This ability is used frequently to fend off the effects of antibiotics and antibacterial;…

Cresyl Violet (CV) staining protocol used in the laboratory. Cresyl Violet is a dye used to stain brain tissues and it is commonly used in histological sectioning. The dye is basic and therefore it binds to ribosomes that are RNA rich and nuclei of cells, which are acidic. Also, the dye leaves a purple color on the stained neuronal tissues. For the purpose of most of our studies, Cresyl Violet staining is used to analyze the lesion size and location in the brain of mice. This staining is…

Identification of Unknown Bacterium #8 Report. Aim: The objective of this report is to identify the unknown bacteria by completing different tests. The test includes things such as a Nutrient agar plate, MacConkey’s agar plate, Gram staining, Oxidase test, UTI ID Membrane, and Eosin Ethylene Blue Agar. Materials: Testing Unknown Bacteria #8 Materials: 1. Nutrient Agar Plate (NA) 9. Hydrogen Peroxide - 2 g/ml. MacConkey’s Agar Plate (MAC) 10. UTI filter 3. Eosin Methylene Blue Agar Plate (EMB)…

The most common bacterial infections that are gram positive are staphylococci which produce enzymes such as catalase which break down hydrogen peroxide bonds, bacillus cereus - food poisoning, bacillus anthracis - anthrax and clostridium tetani - tetanus. Penicillin is an antibiotic that prevents gram positive bacteria from forming peptidoglycan. Peptidoglycan plays a key role in the cell wall. If peptidoglycan was not present, then the bacterium would swell and burst due to the high internal…

manual was conducted to utilize the various morphological characteristics of organisms for the identification of unknown broth culture sample #26. Staining techniques including the gram stain and capsule stain were applied to initially describe the sample as a gram-positive staphylococci bacterium. Upon further examination and comparison with the gram-positive control, the microorganism was correctly described as having ovoid-shaped streptococci cell morphology, implicating that sample #26 was…

Discovering Microbes In A Mini Ecosystem Introduction: Every ecological environment provides with distinct nutrients that allows certain microorganism to survive and grow. Sergei Winogradsky, a Russian microbiologist, developed the Winogradsky Column to grow and investigate microorganisms in a complex biofilm communities. This column will grow different microbes at different gradients that will appear on the column. Depending on the various concentrations of nutrients, choice of soil and water…

spectrum antibiotics. This will be tested by adding the bacteria to a small area on two agar plates – one plate with a gram negative ESKAPE pathogen and the other with a gram positive ESKAPE pathogen. If the bacteria grows on both plates and has a zone of clearance on both plates then it is likely that the bacteria is effective in broad spectrum against both gram negative and gram positive bacteria; this would be the most effective and most helpful type of antibiotic to…