Gram staining

López, C. Sánchez, R. Batlle, and C. Nerín from Spain in 2005, they conducted the antimicrobial activity of clove on several gram negative bacterium E. Coli, Yersinia enterocolitica,Salmonella choleraesuis, Pseudomonas aeruginosa, and several gram positive bacterium, S.aureus, Bacillus cereus, Entero faecalis, and Listeria monocytogenes. The clove essential oil was supplied by ARTIBAL. The compounds in clove essential oil was identified…

the results from the KOH string test. As you can see all test show that the bacteria is gram-positive. The KOH string test is supposed to determine either the bacteria is classified as gram-positive or gram-negative (Sadava, 2011). We dropped KOH with our bacteria and mixed it. After about a minute, we should have been able to tell if a string has formed or not. We have didn’t have a string form, so we had gram-positive bacteria and a negative string test result. We then did other tests…

drug receives three seasons viz. monsoon, winter and summer in months of August, December and May. This serves a rationale to our study, where we find monsoon (August) to accumulate higher levels of CPT (1.337 gram / 100 gram dry bark powder) in barks of Nothapodytes nimmoniana J. Graham. As compared to summer…

β-mercatpoethanol (mol. wt. 78.13) from LOBA Chemie, sodium dodecyl sulfate (SDS) (mol. wt. 288.38) and urea (mol. wt. 60.06) was purchased from Merck, and ethanol was purchased from Sigma-Aldrich. Antimicrobial strain of gram negative bacteria Escherichia coli (E. coli) [MTCC 118] and gram positive bacteria Bacillus subtilis (B. subtilis) [MTCC 121] was purchased from MTCC Chandigarh, India. 2.1. Fabrication of Porous Scaffold from Keratin/Alginate binary blend: The extraction of keratin from…

is essential (Bush 2014). One recent study shows 50% of HIV/AIDS patients had severe sepsis at ICU admission or during the ICU stay. An astounding 90% of nosocomial infections were the source of sepsis and the microbiology of infections was mostly gram-negative rods Serratia marcescens (three) made up 7% (Japiassú…

Velocity of Bacteria and Cells Determine the Need of Antibiotics The first goal was to examine the motion of E. coli bacteria. Excel was used to analyze and record the position, velocity and acceleration of the bacteria. The second goal was to analyze and compare the cell motion of wound healing, neutrophils and E.coli bacteria using ImageJ to determine if a patient needs to take antibiotics to cure his injury based on the velocity of E. coli, neutrophils, and wound healing. Experimental…

As transdermal delivery is a non-invasive route for the therapeutic materials delivery from the intact skin towards the systemic circulation. And as every square centimeter of the surface of the human skin contains ≈10–70 hair follicles and ≈200–250 sweat glands, hence the skin surface could be a conduit for the delivery of therapeutic agents. Additional benefits of using transdermal route is being a painless delivery method, few side effects, providing a low-frequency of therapy administration,…

Type IV pili are a family of appendage protein filaments that are assembled on the surfaces of many different microorganisms, such as bacteria and archea. The wide range of diverse microorganisms within the type IV pili family indicates that there must be both a common evolutionary origin, as well as a versatile functionality (Giltner, Nguyen and Burrows 2012). The formation of type IV pili occurs when the enzyme PilD, first recognized in the bacterial species Pseudomonas aeruginosa, catalyzes…

aureus, S. saphyophyticus, and S. epidermidis all have a similar microscopic appearance, which are gram positive, coccus irregular clusters of bacteria. Additionally, Exercise 27 focuses on an unknown bacterium. Since numerous kinds of microorganisms cultivate in and on the human body as normal microbiota, proper identification is required. Identifying…

containing: one Gram positive bacterium, one Gram-negative paracolon, and one Gram-negative coliform (Carson, 2016). The unknown sample assigned in this experiment was #19. After initially streaking the solution on clean streak plates and TSA agar, the identity was determined from a Gram-stain of a single, isolated colony; this was confirmed by isolation of that pure colony and viewing the pure sample in a Gram-stain under a microscope yielding a blue colored cocci morphology with no…