Gram staining

were several types of antibiotics that have a structure similar to that of a crown ether and one antibiotic that has a structure similar to crown ether would be gramicidin. Gramicidin works against gram-positive bacteria but it doesn’t work against gram-positive bacilli. It also works against select gram-negative organisms. In the year 1939, René Dubos discovered the gramicidin antibiotic.1 Gramicidin contains a mixture of three antibiotic compounds, gramicidins A, B and C and with its…

been working on ways to learn about how Clostridium tetani functions in order prevent Tetanus from being a threat to humans. Causal Agent Clostridium tetani falls under the monera family because it is a bacteria. It is characterized as a “slender, gram-positive, anaerobic rod that may develop a…

In the summer of 2008 Canadian healthcare professionals noticed an irregularly high incidence of listeriosis cases (“Listeriosis outbreak timeline”, 2009), the disease associated with infection by Listeria. This concern was disregarded until people in a nursing home fell ill and sandwich meat from the same place tested positive for the presence of Listeria monocytogenes (“Listeriosis outbreak timeline”, 2009). On August 12, 2008, the Canadian Food Inspection Agency launched an investigation of…

Jaqueline Moreira Biology 23 March 8, 2016 Pseudomonas aeruginosa Introduction P. aeruginosa belongs to the family called Pseudomonadaceau ( Willey, J., Prescott Microbiology, 2014, p.527). P. aeruginosa is a gram negative bacterium. The shape of this particular bacterium is straight or slightly curved rods, but not helical. The size of this bacterium is about 0.5-1.0 micrometers wide and 1.5-5.0 micrometers long. The arrangement is single, but as is starts to age it forms cysts. P.…

highly infectious disease that normally affects herbivores (livestock) and unfortunately may also spread to humans through inhalation, ingestion or any sort of skin contact of the bacterium, Bacillus anthracis. Bacillus anthracis (B. anthracis) is a gram positive spore forming bacterium that invades the human body and leads to various symptoms of Anthrax, depending on how virulent it has become. These symptoms include; swollen neck glands, bloody diarrhoea, vomiting blood, extreme tiredness and…

In Gram negative bacteria cadmium is targeted on DsbA gene in the periplasm. DsbA gene is responsible for the formation of disulfide. Cadmium resistance in some gram-positive bacteria was also found to be mediated by CadA-like proteins (Liu et al., 1997). In S. cerevisiae, cadmium is bound by glutathione, and the resulting cadmium-bis-glutathione…

Biotechnology and probiotic GOS (Galacto-oligo-saccharides) INTRODUCTION What are Prebiotics? Prebiotics are commonly defined as non- digestible polysaccharides and oligosaccharides. These prebiotics enhance the growth of beneficial bacteria like lactic acid bacteria in intestinal tract and also exert antagonistic property against some other species like salmonella sp. or E. coli. Some scientist also defined other properties of prebiotics; which includes resistance to gastric activity,…

In this experimental research, to study the antibacterial properties of the extract of Callistemon viminalis, the standard strains of these bacteria, which are common in urinary tract infections, have been prepared in a lyophilized way in the microbiology laboratory of Fasa University of Medical Sciences: Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis ATCC 29212, S.saprophyticus ATCC 15305, Enterococcus fecalis ATCC 29212, Proteus vulgaris PTCC 1079, Klebsiella pneumoniae ATCC…

2.5. ANTIOXIDANT STATUS 2.5.1. TLC - Semi Quantitative DPPH Assay 0.2 % DPPH solution in methanol was prepared and kept in the fridge for further use. The grid space was marked with 1.0 cm2 space on an aluminum based TLC sheet (Merck silica gel 60F254) and a stock solution of all the extracts together with the standard were prepared in methanol. A series of dilutions of the stock together with the standard were prepared ranging from 400 µL to 0.01µL for the last dilution. The grid on the TLC…

Discussion: The isolate studied was tested for innate antibiotic function on the following media: AC, LB, and 10% TSA. The ESKAPE relative strain that presented a response to the isolate was B.subtilis. In all cases except for that of LB, the isolate showed antibiotic qualities towards B.subtilis by presenting a zone of clearance on AC and 10% TSA media. This behavior is shown in Figures 4, 5, and 6.In the case with LB, B.subtilis inhibited the growth of the isolate as depicted by the empty…