Gram staining

The preparation for this lab should be done a week prior to examining each layer. The preparation consists of compiling the pond. To do so, obtain a small, disposable bowl with a cover. Using a thumbtack, poke holes into the lid of the container. Then, using black paper, wrap it 2/3 of the way around the bowl and proceed to the securing the black paper with aluminum foil. Once that’s completed, fill the bowl 1/4 way with soil and hay. Next, the bowl is filled with water 3/4 way. Cover the bowl,…

Lab Five: Microscope and Simple Staining Introduction A microscope is a tool to examine enlarges image of small objects that are hard to study with the naked eyes. The light microscope, which was used in this lab experiment, is an instrument with two lenses and various knobs to focus the image. We will learn about the proper way to use and handle the microscope. Microorganisms are measured with an ocular micrometer, which is inserted into one of the microscope eyepiece. Micrometer serves as a…

disease/infection properly. Bacteria are classified and identified to distinguish among strains and to group them by criteria of interest (1). There are several different procedures that can be utilized to identify a bacterium. This includes the oxidation test, gram stain, and catalase test. The purpose of this lab report is to show the understanding of all procedures used to identify the bacterium, and explain how the given results led to the identification of the unknown bacterium. Cell and…

previous section we found the following results. The effect of acceleration under the influence of additional mass. Data from our force lab. Column 1-3 shows the results gathered in the first trial from 50, 20 and 10 gram weights. Column 4-6 shows the results gathered from 50, 20 and 10 gram weights when an additional 500g was added to the…

The lab instructor issued out a test tube labeled with letter ‘I’, which consisted of two unknown bacteria, Gram-positive or Gram-negative that were streaked from a pure culture. Sterile techniques were followed while performing precise instructions as stated in the referenced Laboratory Manual. Example 1: The first procedure performed was done by isolating a pure culture from the mixture onto a solid Trypticase soy agar (TSA) media. Sterile technique was done by flaming the loop until it…

Organisms present on the Gram film were obtained from patient sputum. This suggests that the organisms can be present either in the airways or in the lungs. Gram film shows Gram-positive (purple- dark colour) elongated oval shaped (cocci) cells. Bacteria are present as diplococci (pairs of cocci), nevertheless they also appeared as single cells and short chains of few cells (four cells chain is visible at the Gram film). Morphological appearance of bacteria, Gram stain and presence of the…

The possibilities of two unknown microorganisms lead to phenylalanine deaminase test. The detection of phenylalanine deaminase enzyme was carried out by adding ferric chloride to phenyaline slant. When ferric chloride was added to phenylalanine deaminase tube, it turned into army green color( positive result) which indicated that unknown microorganism contains phenylalanine deaminase enzyme. On the other hand, no color change in the slant indicates negative result for this test. The choices…

During the first lab period, The gram stain show that the unknown bacteria was a gram neative rod shaped microbe. After this step was established, this next part was to select the best tests and mediums to determine the unknown bacteria.This medium are use to test the metabolic characteristics of the unknown bacteria. In order to identify the unknown bacteria from other bacterias the following medium was used: MSA plate, SIM plate, nitrate reduction test Nutrient gelatin and Urease broth. For…

reagent in the nitrate broth to see if the color would change. After doing every experiment, it appeared that the nitrate color was red and the citric agar turned blue while we identified our unknown bacterium as Enterobacter cloacae, a clinically gram negative, facultative-anaerobic, rod shaped and motile bacterium. E. cloacae infections can include bacteremia, lower respiratory tract infections, skin and soft-tissue infections, urinary tract infections (UTIs), endocarditis, intra-abdominal…

the gram reaction of the unknown, a Gram stain was done. This process differentiates between a Gram-positive and Gram-negative microorganism. This is done in four steps. The microorganism was stained with iodine, crystal violet, alcohol and acetone, and safranin. Iodine was used first as it acts as a mordant. It forms an insoluble complex by binding with the primary stain. It is then stained with crystal violet. Alcohol and acetone acts as a decolorizer. A distinct feature between both Gram…