Gram-positive bacteria

ureae. The isolation process was performed by streaking a mixed culture onto a nutrient agar plate, then transferring the isolated cultures after incubation for biochemical testing. S. ureae is discussed below. Biography S. ureae is a coccus, gram-positive, endospore-forming, chemoorganotrophic, obligate aerobe and has motility via flagella. (Sporosarcine, 2006). It is typically found in soils at the bases of trees where dogs have urinated, and has also been found in manure (Dworkin,…

Second Rule” Affect the Amount of Bacteria on Food? Background Information Many people have heard of the “five second rule,” a saying that refers to the common belief that if one drops a piece of food on a floor or other surface, germs will not have time to adhere to the food if picked up within five seconds. This idea is supported by Hilton (2014) who did research and found that “food picked up just a few seconds after being dropped is less likely to contain bacteria than if it is left for…

microbes to adapt. The most common medical issues treated by antibiotics are: respiratory-revolved, upper respiratory tract infections, urinary tract infections, and ear infections; in many cases, these are frequently caused by viruses rather than bacteria and are not even treatable by antibiotics. There were 23,000,000 prescriptions overall for these four complications (Antibiotic Resistance Research and Surveillance, 2014). Also, large amounts of antibiotics are introduced into the…

Water and Iodine. The procedure of the lab for the first day involved our group acquiring a cotton swab and using the swab to gather the Propionibacterium acnes (P. acnes) bacteria from various spots around our face, we then grabbed a Petri dish filled only with a thin layer of agar. We proceeded to swab the P. acnes bacteria off of the cotton swab (gently to not break the agar) into the Petri dish. Once we had completed swabbing the P. acnes over the agar we closed the Petri dish, taped over…

Throughout the course of the semester we have learned how different variables in an environment can affect the growth, change, and behavior of an organism. Using this research as a guide we set out to design an experiment that would show a particular variable’s effect on brine shrimp eggs. After much debate we decided to use light wavelengths as the variable being tested. We wanted to see the difference in development in the brine shrimp eggs and the development after hatching when…

evaluated for their anti-microbial activity using the agar diffusion technique . The tested compounds were evaluated against, Gram +ve bacteria , Gram -ve bacteria, Yeast and Filamentous Fungus. Minimum inhibitory concentration (MIC) was made in vitro for compounds which showed antimicrobial activity. Compound 3b has broad spectrum activity against Gram +ve bacteria, Gram -ve bacteria, Yeast and Filamentous Fungus with MICs 150, 150, 75, 312, 625 and 625 µg/mL against (Bacillus subtilis…

uncountable. To ensure a countable plate, serial dilutions of a solution containing an unknown amount of bacteria are plated. The total number of bacteria in the original sample can be determined by counting the colony forming units and dividing them by the product of the dilution factor and the volume of the plated diluted suspension. This formula helps us to calculated the number of bacteria per mL present in the original solution.…

There are bacteria everywhere. Bacteria is on everything that we touch, eat, and is in our own bodies. We cannot see them with just the naked eye, it takes a microscope to be able to see them. Even though they are visible through a microscope, it is not possible to tell what they are. Two ways that we can identify bacteria is by using agars and staining. The agars that are used in this lab are eosin methylene blue agar (EMB), mannitol agar, and nutrient agar. Mannitol agar is used for…

What is biodiversity? Introduction Biodiversity is defined as the variety of life on Earth at all its levels and includes genes to ecosystems, as well as the ecological and evolutionary processes that sustain it (Cardinale, 2012). The Earth’s biodiversity has long been a source of wonderment and scientific curiosity, but is increasingly becoming a source of concern. The sheer diversity of life is of inestimable value. Biodiversity has a number of functions on Earth, from providing a foundation…

of food poisoning caused by the microorganism Clostridium botulinum. Clostridium botulinum is a gram-positive bacterium, bacillus in shape, and is an obligate anaerobe.1 The bacterium can produce endospores, making it resistant to microbial control methods. Clostridium botulinum survives in nonliving reservoirs1 . It is commonly found in soil and large bodies of water. The classification of the bacteria is shown below in table 1. Clostridium botulinum is very harmful to humans in that it can be…