the mixed unknown bacteria was then passed over the flame. The inoculating loop was then inserted into the broth and then carefully streak on quadrant one of the plate. Afterwards, the loop was sterilized by the flame and was allow to cool for thirty seconds so the next streak can be applied. The same concept was then applied for the different metabolic test. It is important to practice aseptic techniques to prevent contamination of results (3). A broth of mixed unknown bacteria was given and…
Wescodyne for both bacteria was over 20 mm. Only Wescodyne was able to inhibit both bacteria. E. coli was inhibited by hydrogen peroxide and 70% ethyl alcohol. S. aureus was inhibited by Lysol and Scope. b. The tests that were performed were not effective in distinguishing between bacteriostatic and bactericidal agents. The zone of inhibition may have been a result of both bacteriostatic and/or bactericidal properties. The disinfectants/antiseptics may have prohibited the bacteria from growing…
Gram-positive cocci are an interesting group of bacteria to study. Some common species include Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Streptococcus pyogenes, and Sarcina lutea. Gram-positive cocci have a gram positive cell wall, cocci morphology and many different styles of grouping. Some species have begun to exhibit a resistance to fluoroquinolone by the use of altering the drug binding site and the use of a membrane pump (Hooper 2002). Thanks to the…
Bacteria also known as prokaryotes are very ancient single-celled organisms; fossils show that they were widespread 1.5 billion years ago. Today bacteria make up the vast majority of prokaryotes on earth due to the unfavorable ability to adapt quickly to the surrounding environment. Bacteria have the capabilities to survive in areas of high heat, extreme cold, or even very acidic or alkaline conditions. This ability is used frequently to fend off the effects of antibiotics and antibacterial;…
main topics could be the drug resistance mechanisms of bacteria. 2. First, spontaneous mutation which changes the drug target or upregulate drug efflux. Second, Horizontal gene transfer (HGT) that the genes in bacteria acquire to become resistant. HGT can cause multidrug resistance in a genetic event which make the bacteria become resistance. Third, the ability of bacteria that they can form biofilm while they are being infected. The bacteria provide themselves protection to against the drug…
spots where the sunlight was hitting the column. Meanwhile, the experimental presented less green bacteria. The black spots increased in both columns, but the experimental group had a greater area of black spots. Finally, the experimental group had some orange spots which means that the column had non-sulfur bacteria, and above the orange layer, there was a big green spot of green non-sulfur bacteria look figure 1.3 (Urmeneta & Duró, 2011). The orange and green appeared in the opposite site of…
General Unknowns Bacterium Vitality en Vida Bacteria play a vital role in our daily lives. It is therefore our right to know the importance of being able to distinguish certain strains of bacteria. With the right knowledge we would be able to come up with a cure for a disease. An identity gives way to knowledge, to better understanding and access to information on how an organism works and how we can prevent its worsening effects or isolate its benefits. Scientists have been trying to…
Most people also tolerate penicillin very well, though a small number of people are allergic ("Penicillin allergy - Symptoms and causes", 2018). As you may know, bacterial cells have cell walls containing peptidoglycan. When bacteria divide, holes actually appear in their cell walls that must be filled with peptidoglycan. However, penicillin inhibits proteins that are needed to link the peptidoglycan together. So when bacterial cells try to divide when penicillin is present, the holes in the…
yellow, indicating a positive result. A negative result is indicated by no color change or a change to deep magenta if ammonia is produced. Another form of a positive result is the formation of a gas bubble in the Durham tube. This result will usually occur along with the acid…
of settings; from clinical diagnostics to environmental monitoring (1). Species of bacteria can differ fundamentally in aspects ranging from morphology to metabolism. Exploiting these differences can allow for identification of unknown organisms. Gross morphological characterizations are crude, and better replaced by more accurate and specific tests. At a fundamental level, tests such as the Gram stain divide bacteria in to two broad categories based on the characteristics of their cell wall…