inhibition zone. Bacteria are tiny microorganisms that colonise together and are found in every living organism. Bacteria is a type of Prokaryotes and RESEARCH Some Bacteria can be extremely harmful to many animals bodies including humans, these germs attack the immune systems and cause sickness and diseases, these illnesses occasionally result in death. Although not all bacteria are…
from a member of our group’s hand. Bacteria are prokaryotic, but like all other living organism there are many genus and species. Through experimentation these unknowns can be found can be brought to light. Bacteria can be classified either gram-positive or gram-negative. These groups differ by the composition of their cell walls. In particular, the difference in the composition pertains to amount of peptidoglycan, a polymer of amino acids and sugars, present. A Gram test is most commonly used…
The purpose of the research was to potentially find a new bacteria that is capable of producing an antibiotic against one or more of the six ESKAPE pathogens (a group of pathogens that are resistant to most if not all current antibiotics). All research protocol was based on the Yale Small World Initiative and the third edition of Microbiology Laboratory Theory & Application. One bacteria from a sample of soil collected (colony #18) was tested for its ability to produce antibiotics against two of…
Broth culture #20 was selected and subjected to qualitative tests for identification. Gram stain tests were performed in order to identify which unknown is gram positive and gram negative. Using selective and differential media, like MacConkey agar which allows the growth of gram negative bacteria only that are able to ferment lactose. Also, mannitol agar was used which isolates and detects gram-positive bacteria. As well as blood agar, which is a nutritive media with differential properties in…
Once the bacteria was on the agar, we closed the lid immediately, hoping to prevent microorganisms in the air from contaminating our sample. We also collected a sample from the skin of a lab member, and another from the microorganisms in the air. Once our samples were placed in the agar,…
unknown has a gram-negative envelope which stains red or gram positive envelope which stains blue. Gram-negative microorganisms stain red because of their inability to bind crystal violet and their ability to bind the safranin. Gram-positive microorganism, however, stains blue due to their ability to retain the primary stain, crystal violet. I determined my unknown sample to be a gram-negative microorganism. After viewing the slide under the microscope, I concluded that my unknown gram-negative…
was identified as a gram negative bacteria. The biochemical tests that I performed that were positive were catalase, glucose, SIM, indole, citrate, and urea. First, the catalase test has the presence of an enzyme in the test isolated detected using hydrogen peroxide. Bacteria possess catalase with a small number of bacteria; isolation is added to hydrogen peroxide when bubbles occur. When the bubbles occurred on gram positive bacterium it was a conformation of being positive. Next, the glucose…
suggests that the difference between the two may not be significant (Figure 2). These results support part of the hypothesis as the two species of bacteria developed different zones of inhibition when exposed to the three antibiotics. Gram-staining confirmed the identity of S. epidermidis as a gram-positive bacteria and S. enteritidis as a gram-negative bacteria.…
1. The biggest potential source of contamination of the locations we tested in the Distribution of Microorganisms would have to be the floor of the lab. The reason why this is the biggest potential source of contamination is because multiple people are coming in everyday from the outside world, bringing in microorganisms from the outside into the lab. And although we sanitize the counter and all the spaces that we work around, we don’t take precautions to necessarily clean the floor, making it…
Reproduction of Archaebacteria Archaeal bacteria do not have a nucleus, so it can not go through mitosis. In a means to reproduce, they procreate using binary fission. During the process multiple fission, the replication of the archaeal DNA occurs and the two strands are pulled apart. Archaeal chromosomes replicate using DNA polymerases that mirror complements eukaryotic enzymes. Protein FtsZ, the protein that directs cell division, forms a contracting ring around the cell. The factors of the…