Gram-positive bacteria

contained two unknown bacterial cells and they either Gram-negative or Gram-positive. The procedures were followed as stated from the course laboratory manual by Meramec Community College (1). The first step was figuring out the unknowns was to separate the two bacteria. In order to do this, the Trypticase Soy agar was used. The streaking method was used to spread the bacteria across the Trypticase Soy agar to isolate the bacteria. In order to do the streaking method, an inoculating…

be performed was a gram stain. Gram staining determines whether the microorganism is gram positive or gram negative. After doing these procedures it was determined that the organism was a gram positive cocci. The biochemical test performed were chosen based on this information. Principles of biochemical test: Test Reagents Purpose Slant Shows Growth Gelatin deep Shows Motility and production of…

1 1. Bacteria is stained using a primary stain called crystal violet and then fixed with a mordant which in most cases is iodine. While some bacteria have the ability to retain the primary stain, others discolor when exposed to the acetone and alcohol (95%). Hence, the Gram stain divides bacteria into two groups- these bacteria are grouped according to their differential staining properties. One group is Gram positive bacteria and the other group is Gram negative bacteria. The Gram positive…

ideal staining for identifying a Gram positive bacteria – Please explain your answer? The ideal staining for identifying a Gram positive bacteria is brown-brenn gram stain is preferred to detect the positive bacteria. 13. What is the ideal staining for identifying a Gram negative bacteria – Please explain your answer? The ideal staining for identifying a Gram negative bacteria is the brown-brenn modification is superior for the detection of the gram negative bacteria. 14. How does…

and Identification of an Environmental Bacteria to the Genus level Introduction: Bacteria can be found virtually anywhere, and interaction with these organisms occurs daily (Fitzpatrick & Allison, 2014). However, though the interaction between human and bacterium is continual, it is not always known what type of bacteria a person is coming into contact with. Therefore it is important to understand, and to have knowledge about, the characteristics of bacteria in our environment. As part of a…

Gram staining was used to distinguish the unknown microbes between gram-positive microbes from gram-negative microbes. Gram-positive microbes contain a thick peptidoglycan with only one outer cell membrane, thus dying the microbes dark purple. Gram-negative microbes contain a thin peptidoglycan with an outer and inner cell membrane, dying the microbes pink when a gram stain test is done. Microbes A and C were gram- positive because of purple dye seen under the microscope. Microbe B was a…

wall helps to differentiate major types of bacteria” (Tortora, Funke, and Case 84). The two main types of bacteria are gram positive and gram negative. Their cell walls are composed of peptidoglycan. The peptidoglycan is a chain of repeating disaccharide held together by polypeptides. The disaccharide…

plate showed growth of two different colonies. After the morphology was observed and recorded, a Gram stain was performed on each of the two unknowns, this procedure is found in the laboratory manual. The two microorganisms were identified; one was a Gram-positive rod bacteria, the other was a Gram-negative rod bacteria. A series of biochemical tests were performed based on the results of the Gram stains. The proper procedure…

world is composed of many bacteria that can either help or destroy us. Therefore, its’s imperative to learn and study them. The purpose of the lab was to put into action the methods learned in the laboratory to determine our unknown bacteria. Bacteria can have different features, shapes, and or arrangements that help microbiologist differentiate them. Bacteria can be classified as Gram positive or negative (difference in cell wall), with the help of the Gam stain (“Gram Stain”, n. d). Another…

The objective of this lab was to isolate and identify an unknown Gram positive and Gram negative bacteria through the use of various microbiological techniques. The identification of the genus and species for the unknown culture should be obtained based on the characteristics for Gram positive and Gram negative bacteria. Bacteria are prokaryotic microorganisms that lack a nucleus and therefore all its genetic information is stored in the cytoplasm of the cell. The bacterial cell envelope…