Gram-negative bacteria

catheter specimen it is collect from the catheter and not from the collection bag. Most of these stains are lactose negative and they demonstrate typical features swarming in motility on agar plates. According to Smith (1993) swarming is the spreading of Proteus…

lab were used due to the fact that some tests were not listed on the manual, while others did not apply to the category of the bacteria. Test used to find the morphology characteristics of the unknown yielded that the colony of the unknown was yellow pigmented and the shape of the cell was rod. More information was found by examine the cell wall structure of the bacteria, Gram test revealed…

the gram reaction of the unknown, a Gram stain was done. This process differentiates between a Gram-positive and Gram-negative microorganism. This is done in four steps. The microorganism was stained with iodine, crystal violet, alcohol and acetone, and safranin. Iodine was used first as it acts as a mordant. It forms an insoluble complex by binding with the primary stain. It is then stained with crystal violet. Alcohol and acetone acts as a decolorizer. A distinct feature between both Gram…

for identification. A Gram stain was done it reveal a gram positive cocci result. A streak plate was completed revealing that the microbe was round/filamentous, convex, erose, granular, yellow, and opaque. The next test was the Tryptic Soy Agar plate for color. Followed by the oxidase test and last was the Mannitol Salt Agar test. Thus microbe #1R was Sporosarcina ureae (Brady, Personal Communication). Procedure of the Gram stain Purpose: The Gram stain reveals whether…

various methods. Most stains used in microbiology are differential stain, which use more than one dye so that different cells, chemicals, or structures such as Gram staining, TTC reduction, Oxygen requirement test, Catalase test, Nitrate test, Motility, Triple Sugar test, Carbohydrate Fermentation test, and the IMViC test. Especially, the Gram stain differentiates between two large groups of microorganisms. Plan 03-05-16 The Unknown I.D. Project determines identification of a…

the disease it causes. At the beginning of class, the TA handed each student an agar plate containing some unknown bacteria, each one was numbered. Mine was number 14. The teacher then asked the students to use the techniques used in class (sterilize the inoculated loop, get a small amount of the unknown bacteria, incubate) to identify the unknown bacteria. To start, the unknown bacteria had to be successfully isolated. Using a wire loop, a small sample of the numbered test tube was plated on…

polysaccharides and oligosaccharides. These prebiotics enhance the growth of beneficial bacteria like lactic acid bacteria in intestinal tract and also exert antagonistic property against some other species like salmonella sp. or E. coli. Some scientist also defined other properties of prebiotics; which includes resistance to gastric activity, stimulation of some selective growth, and enhancement of growth of intestinal bacteria. There exist a number of prebiotics which are classified on the…

of P4 8 ml/kg of feed to give the maximum results, it is presumed the necessary bacteria concentration too much then it will give rise to overgrowth. According to the Atlas and Richard (1993) that the density of…

The objective of work was to optimize, formulate, evaluate and compare the transdermal gel containing vesicle based: Transfersomes and liposomes, containing Ketoconazole, an anti-fungal bioactive. Ketoconazole loaded Transfersomes were prepared using thin film hydration method with different concentration of surfactant (Span80, Tween80, and Sodium Deoxycholate) and soya lecithin and characterized for various parameters including Vesicles Size, Morphology, Entrapment Efficiency, Drug Loading…

Bacteria They are formed by a single rudimentary cell. They have no nucleus or plastids, but chromatin. Some have assimilators pigments. They are on earth, water, and air, and can generally move. They may be causing disease and therefore are called pathogens. Those that do not cause disease are called saprophytes. In some cases, they are even useful, as in digestive processes or synthesis of vitamins. By the way, they are called coconuts when circular; bacilli if they have the form of rods;…