Gram-negative bacteria

Organisms present on the Gram film were obtained from patient sputum. This suggests that the organisms can be present either in the airways or in the lungs. Gram film shows Gram-positive (purple- dark colour) elongated oval shaped (cocci) cells. Bacteria are present as diplococci (pairs of cocci), nevertheless they also appeared as single cells and short chains of few cells (four cells chain is visible at the Gram film). Morphological appearance of bacteria, Gram stain and presence of the…

consists of a single dye which informs only about cell shape and size, rather than differences between cell components. Through the simple stain, it was found that the unknown is a staphylococcus microorganism which is spherical and in clusters. The gram stain was performed in laboratory six and this helped determine whether the…

were performed that were specific to the gram- negative bacteria to start the identification process of the unknown. The results of the gram negative tests lead to the identification of the bacteria Alcaligenes faecalis. The gram stain procedure was the first test performed by isolating a colony from the pure culture streak plate. The gram stain was followed orderly right from the referenced laboratory manual. The test resulted to be Gram- Negative bacteria from the compound microscope which…

Gram Staining Procedure Doctors often want to determine what type of bacteria is present in a patient who has an infection. Aside from other tests, experts use gram staining procedure as one of the techniques to differentiate bacteria according to their cell wall characteristics. It involves using a dye to see which bacteria retain either color (violet or red) in their cell walls. The importance of using the gram staining procedure is related to the choice of treatment for the patient. Here is…

and I were given unknown number 3 in the laboratory. After performing various tests over the course of a few weeks on our unknown, we came to the conclusion that our unknown organism is Klebsiella pneumoniae (K. pneumoniae). K. pneumoniae is a gram negative bacillus shaped microorganism. We observed that K. pneumoniae is a nonmotile organism. We performed multiple tests on our unknown culture, therefore we are very confident that it is correctly identified. We identified that K. pneumoniae is…

During the first lab period, The gram stain show that the unknown bacteria was a gram neative rod shaped microbe. After this step was established, this next part was to select the best tests and mediums to determine the unknown bacteria.This medium are use to test the metabolic characteristics of the unknown bacteria. In order to identify the unknown bacteria from other bacterias the following medium was used: MSA plate, SIM plate, nitrate reduction test Nutrient gelatin and Urease broth. For…

Question: How much bacteria will be found in each pedicure spa? Hypothesis: If I swab multiple pedicure spas using a test kit, streak plating, and colony morphology, then I will find the presence of bacteria is unsafe because cleaning practices are not followed properly. Procedures Determine the five pedicure spas in which you will obtain samples from Give each pedicure spa an anonymous name (Ex: Spa A, Spa B, Spa C) Type up an approval letter stating what you will be doing and what it is for…

The purpose of the infections caused by gram-positive cocci lab exercise is to utilize different identification and diagnostic techniques to differentiate various groups of bacteria, which cause infection. Our primary goal is first to establish the presence of an infection; then, determine which particular pathogens, or groups of pathogens, are the underlying cause of the infection. In the clinic, infections manifesting from gram-positive cocci are a common and growing problem. Two genera in…

needed to do to get started was to do a gram stain. This would help me decide which kind of further testing would need to be done in order to help identify the organism that is causing gastrointestinal distress in Brown County. After doing a gram stain test on my unknown, I found that it was a gram negative organism. This prompted me to do some tests that are specifically for gram negative organisms. When I looked under the microscope I seen some rod shaped bacteria. So what is this organism and…

Proskauer Broth tubes. The tubes will be then incubated at 35°C for 48 hours. One tube will be placed with five drops of methyl red reagent, and the other with Barrit’s reagent. Methyl Red test is used for the indication of fermenting ability of bacteria, producing mixed acid. Voges-Proskauer test is used to detect the production of acetoin, also known as 2,3-butanediol. 6. Indole – Motility…