Prescott Microbiology, 2014, p.527). P. aeruginosa is a gram negative bacterium. The shape of this particular bacterium is straight or slightly curved rods, but not helical. The size of this bacterium is about 0.5-1.0 micrometers wide and 1.5-5.0 micrometers long. The arrangement is single, but as is starts to age it forms cysts. P. aeruginosa is an aerobic bacterium with oxygen being the last terminal electron acceptor. However, this bacteria can also grow without the presence of oxygen by…
first isolated by Alexander Ogston. Ogston described the bacteria, which he isolated from pus collected from surgical wounds, as micrococci (Thomer et al., 2015). This description was given because of the sphere-shape of the bacteria and the grape-like clusters that they form. After Ogston’s discovery, Friedrich Rosenbach differentiated between two staphylococci bacteria, which were isolated from humans (Thomer et al., 2015). These bacteria were named S. aureus and S. albus. This nomenclature…
cell. Bacteria live in dilute aqueous environments, which causes an intracellular pressure equivalent of 10-25 atm. Without the structural support of the cell wall, the cell is susceptible to lysis and osmotic rupture due to osmotic rupture due to turgor pressure. Figure 1: Comparison between gram-positive bacterial cell walls and gram-negative bacterial cell walls. Structural differences in the bacterial cell walls cause bacteria to be subcategorized into gram-positive and gram-negative.…
infections it can lead to under specific conditions. Introduction Propionibacterium acnes (P. acnes) is a bacteria that grows deep inside of pores, which is relatively slow-growing, typically aerotolerant anaerobic and gram positive type, It feeds on the sebum that is made by the sebaceous glands which surround the base of hair shaft. One of the most prominent properties of these bacterias is that it’s partially…
Introduction: Bacteria are very diverse and are part of the prokaryotic domain. Some bacteria can be harmful, but others can also be beneficial in many ways. There are bacteria everywhere on Earth; places that you would think would have bacteria, like your gastrointestinal tract or our skin (Wenner, 2007). Although bacteria are small in size and are a simple cell organism, it is essential to life. There are different ways in how to distinguish and classify bacteria. One way is you can look…
timeframe of three weeks to determine the identity of three unknown bacteria contained in broth medium stored in labeled test tubes that were randomly assigned containing: one Gram positive bacterium, one Gram-negative paracolon, and one Gram-negative coliform (Carson, 2016). The unknown sample assigned in this experiment was #19. After initially streaking the solution on clean streak plates and TSA agar, the identity was determined from a Gram-stain of a single, isolated colony; this was…
Identification of bacteria can be a difficult process due to the fact that individual bacterial cells can possess similar structural morphology to other types of bacteria. As we have demonstrated in previous lab experiments there are key biological and chemical processes that are characteristic to different types of bacteria. Using various biochemical tests we can evaluate and detect these different processes to aid in our identification of unknown bacterial samples. The implications of these…
Discussion: The isolate studied was tested for innate antibiotic function on the following media: AC, LB, and 10% TSA. The ESKAPE relative strain that presented a response to the isolate was B.subtilis. In all cases except for that of LB, the isolate showed antibiotic qualities towards B.subtilis by presenting a zone of clearance on AC and 10% TSA media. This behavior is shown in Figures 4, 5, and 6.In the case with LB, B.subtilis inhibited the growth of the isolate as depicted by the empty…
order to determine the identity of two unknown bacteria, I performed a series of experiments over several days. To begin this process I created a dichotomous key that I utilized as a guide to perform a series of inoculations, while using both selective, and differential media. The series of inoculations eliminated bacterium based on test results until the correct bacteria were both positively identified. On day one of this process I received two bacteria, one labeled 7A and the other labeled…
López, C. Sánchez, R. Batlle, and C. Nerín from Spain in 2005, they conducted the antimicrobial activity of clove on several gram negative bacterium E. Coli, Yersinia enterocolitica,Salmonella choleraesuis, Pseudomonas aeruginosa, and several gram positive bacterium, S.aureus, Bacillus cereus, Entero faecalis, and Listeria monocytogenes. The clove essential oil was supplied by ARTIBAL. The compounds in clove essential oil was identified…