Gram-negative bacteria

When trying to figure out the cellular morphology and arrangement the T-streak method is used to get isolated colonies from a sample of a bacteria. To begin the process a petri plate which has a layer of agar is divided into three sections in a T form. When preforming an experiment aseptic techniques are used between each step, therefore you flame the loop before and after every step. A sample is taken from a culture with an inoculated loop, then is spread in the top section of the plate in…

this bacteria can be spread in different modes such as direct contact from an infected wound, contact with the hyaluronidase, an enzyme produced by the bacteria that destroys tissues or even skin to skin contact with an infected person. There are some difference in characteristics between Gram-positive and Gram negative bacteria, where one of most distinctive differences is that Gram-positive bacteria composes of a thick layer of peptidoglycan enveloping the plasma membrane, where as…

unknown bacteria, bacteria #304, it was discovered to be Escherichia coli or for short E.coli. I can conclude that my answer is correct because several tests were conducted leading me to the specific bacteria. The first step taken to discover the unknown bacteria was a gram stain. A section of the unknown bacteria was taken and stained with crystal violet and safrinin so that the bacterium was visible under a microscope. The gram stain gave the opportunity to know whether the bacterium was gram…

about the Bacteria: First, that the bacterium was bacilli (rod) shaped. This was determined by viewing individual cells through a microscope at 400x power. Secondly, that the bacterium was most likely a Gram-stain-negative because it appeared to be a pink color after gram-staining. This had several implications: The experimenters now knew the bacteria’s cell wall composition, its outer membrane composition, peptidoglycan concentration, outer membrane amount, and more. Because of the gram stain…

journey to the microscopic world of bacteria can be likened to a child exploring the world for the first time. As the child discovers the world, she also learns several survival skills along the way. In the same manner, all the knowledge I learned from Microbiology class and laboratory sessions regarding microbial world equipped me to identify bacterial unknowns from a clinical sample. The task was to successfully separate and accurately identify two bacteria of a specimen taken from an…

study was to preform many tests to isolate and identify what types of bacteria inhabiting the sample of forest soil tested. Methods: Many steps were taken to isolate and identify the different types…

growing in a sterile medium, such as agar. The mixed culture can include multiple species of viruses, bacteria and parasites, which may or may not live in harmony with one another, sharing the available resources" (Kathryn Barr, 2016). I took number five test tube from the different microbes that I had the option of choosing from. From that I created a mixed culture for myself and was able to do a gram stain to narrow down my unknown microbes. A mixed culture contains many species that disperse…

Serratia marcescens 1b. Glucose not fermented 8a. Glucose oxidized…………………………….........…………Pseudomonas aeruginosa 8b. Glucose not oxidized…………………………………………......Alcaligenes faecalis Test Question being asked Observations Interpretations Gram Statin Is the unknown gram…

determine two bacteria in a given broth. For this experiment we were provided with an unknown broth, along with the supplies and media to perform the needed tests that would determine what bacteria the broth contained. We were also provided with a chart of possible bacteria. All tests were preformed with aseptic and sterile techniques. After being given the broth, using sterile methods I streaked a TSA plate using the quadrant method with the given broth, in hope to grow the bacteria in…

unknown bacteria in a mixture sample. Also, throughout the project learn about the morphological and physiological characteristics of the bacteria that distinguish them from each other. The methods and testing procedures performed included: T-streak, Gram Stain, Oxidase Test, Catalase Test, and EnteroPluri-Test . Highlighted in the experimental flow chart it states the tests performed and the results that led to the identification of isolate A and isolate B in sample 4-24. Each test…