Gram-negative bacteria

previous section we found the following results. The effect of acceleration under the influence of additional mass. Data from our force lab. Column 1-3 shows the results gathered in the first trial from 50, 20 and 10 gram weights. Column 4-6 shows the results gathered from 50, 20 and 10 gram weights when an additional 500g was added to the…

and Unknown B bacteria’s. These techniques are gram staining and metabolic testing. I first used Gram staining to distinguished and identify the bacteria’s. Han Christian discovered gram staining in 1882, he had biopsy a patient lung that had pneumonia. The main purpose for Gram staining is to identify if is Gram positive or Gram negative by using differential staining. While Gram staining shows the difference between Gram Positive and Gram negative and its size, shape and morphology. This…

Gram + Cocci To identify the Gram + cocci I first performed a catalyst test which produced oxygen bubbles and indicated a positive catalase result. The next test I preformed was a mannitol salt test using a mannitol-salt agar plate and the results showed positive fermentation turning the plate yellow and also positive growth. This led me to conclusion that my unknown Gram + cocci was Staphylococcus aureus. Gram – Rod To identify the unknown Gram – rod I first performed a H2S production test…

Introduction: Klebsiella pneumoniae is a part of the family Enterobacteriaceae. This genus is a gram negative and it has no flagella, which meant that this bacterium is not motile. However, it can move with the use of the current that is produced by the water. This bacterium has the ability to cause destructive change in a man or animals lungs if aspirated. In addition, this is also one of the most contacted pathogen in a Hospital Acquired Infection (HAI) or nosocomial infection. In this…

Gram staining is a technique where certain stains are used to identify bacteria. It was discovered in 1884 by Hans Christian Gram where he used stains to differentiate bacterial cells in diseased lung tissue. Gram staining has evolved over time to become a trusted and common diagnostic tool used in clinical and research settings. Gram stains are mainly used to identify unknown bacteria. It is often the first test used on an unknown sample of bacteria. For medicinal uses gram staining is commonly…

The Gram stain is a valuable tool to microbiologists in identifying the cell well composition of prokaryotic species. The underlying process revolves around a series of chemical dyes, and their propensity to bind to peptidoglycan polymers in the cell wall. The results allow scientists to infer properties about the outer coating of an organism and is often the first step in identifying an unknown species. For the purpose of our experiment, the results of the Gram stain will be as follows: Gram…

correctly identify two unknown bacteria. Organism number thirteen is Escherichia coli. On the first day, I conducted a gram stain and noticed that the bacteria were pink, meaning that the bacteria are gram negative. I also recognized that the bacteria were rod shaped therefore they were bacillus. I also noticed that the arrangement appeared to be single celled. I also put the bacteria on a streak plate. On the second day, I did another gram stain and the bacteria were again pink and bacillus or…

specimen thrive in aqueous environments, therefore they are translucent and when mounted on a microscope the light passes through them making it impossible to see the specimen. Equally as important as this is that the viewer is aware of what type pf bacteria is being utilized. Both of these criteria in a lab are met with the use of a staining procedure which allows for greater contrast between the mostly translucent cells and light used by the microscope. Staining is a critical step when…

Bacteria’s phenotype is commonly described as gram-negative or gram-positive from conducting a gram stain. A bacterium that is gram-negative tends to have cells composed of a thin peptidoglycan layer, and a gram stain of gram-negative bacteria are seen as red or pink through a microscope (Smith and Hussey, 2005). On the other hand, a bacterium that is gram-positive will have cells made of a thick peptidoglycan layer, and the gram stain of a gram negative bacteria would be seen blue or purple…

Gram Negative Unknown Lab Report #20 BIOL 3444-008 Giang Nguyen TA: Malini Kotak 11/05/2014 Abstract The experiment is conducted in order to identify a randomly given unknown gram-negative microorganism. By comparative analysis of biochemical tests that were performed throughout the semester to find out its characteristics and determine the identification of bacterium out of four potential unknown was given. To figure out the characteristics, T-streak was performed to isolate…