P. putida and P. fluorescens isolates were cultured in 250 ml Laurea Broth (LB) medium at 30±1 ºC for 36 h in an incubator shaker at 150 rpm. The culture was then poured into 50 ml falcon tubes and centrifuged for 10 min. at 6000 ×g at 4 ºC. The pellet was washed thrice in 15 ml distilled water and centrifuged. Then it was dissolved in distilled water and the optical density (O.D) of the culture was set at position-1 at 600 nm=107–108 colony forming units (CFU) ml-1 for inoculation (Bhuvaneswari…
significant difference in stomatal densities between young and old leaves or no. The stomatal density of young and old leaves has not significantly different densities. The age of the leaf does not аffect and do not correlate with stomatal density. Because, when the leaf is a baby leaf it makes all the stomata and as leaf growth it just pulls apart from each other. Stomata has an essential rоle in all plаnts. Stomata helps to maintain balance between water and gas exchanges. The word stomatа…
The Quaking aspen (Populus tremuloides) is a very abundant tree in the mountainous regions here in Utah. Moreover, this particular species of tree is very popular because it grows most commonly around burn sites. After a natural disaster the Quaking aspen is usually one of the first trees to take root. The Quaking aspen is well known here because of this, however the common person may find it hard to identify when an aspen tree is not healthy. Sooty bark canker (encoelia pruinosa) is a canker is…
and are guaranteed a percentage of food from the farm, and miners are offered a similar bounty of housing, food, and are given a certain amount of precious metals as well. Food and precious items flow into the economy through trades, a few ounces of gold for a plot of land, or food to last your family a…
Due to today’s media which has sensationalized and embellished the more magical aspects of alchemy, the layman may imagine dark spirits and flasks overflowing with noxious chemicals. The layman may also think of alchemy as a trial and error process, in which the aspiring alchemist fumbles blindly towards his next success or failure. This is far from the truth. The infancy of alchemy is located in a Greece influenced Egypt ("Alchemists, Ancient and Modern."). Ancient Egypt, Alexandria, had…
Even though Midas was a very wealthy king he still wasn’t satisfied with the riches he had, so he asked to have a golden touch, turning everything into gold, literally food and everything he touched, which he soon realized he couldn’t even eat anything without it turning to gold. Loy states, “today this simple yet profound story is even more relevant than it was an ancient Greece”(Loy 26). To bring his point across, I agree with Loy, because today no matter how…
Golden Mountain( San Francisco). When he gets to San Francisco Moon Shadow, his father Windrider, his uncle's, Hand Clap, And Bright Star start a company. The company is a group of people that mine in the California streams for gold. They make a trip to a mountain to get gold with Hand Clap as their guide. They also meet their cousin Lefty. They aren’t allowed to talk to the people of the middle kingdom. Also, the demons…
They both wanted those cherries, but they knew that the man would not just give them the cherries. So they came up with a plan to trick the man into giving them the cherries. They took pieces of wood, carved them to look like coins and painted them gold. Badger and Otter approached the man and ask for the basket of cherries for the coins. The man was hesitant at first, but some money is better than none. The man gave Badger the basket of cherries in exchange for the coins. Badger and Otter ran…
Spirit was all for living in the way of how god wanted. She explains that by living in the puritan lifestyle there was a place that they could acquire. Spirit remarks a place typified as heaven and she describes “the streets are thereof transparent Gold” (224). Coming to the conclusion that whatever the world had there was to be much greater goods in heaven. The recompense would be worth it, if she lived religiously. She later describes them two from being polar opposites. Revealing that they…
personal legends and are instead content with material desires and social acceptability and miss out on various opportunities because of their limited perspectives. alchemy which is a laborious process that transforms basic metals into luxury metals like gold represents santiago’s convoluted journey to achieve his personal legend. in the book the alchemist says to santiago that a base metal must rid itself of all impurities to achieve a higher evolutionary state and in retrospect achieve its…