Genomics

genetics and is utilized for many purposes, which include genes, genotyping, sequencing, cloning, etc. (Kelly, 2016). This allows for the ability to conduct DNA analysis revealing varying compositions and properties. For the following experiment the genomic DNA from Drosophila melanogaster, was utilized to be cloned and sequenced for either actin or 18s rRNA. In order to clone and sequence the specific section of DNA 9 specific steps had to be conducted and they are as follows:…

E. coli Genomic DNA Isolation and Quantitation Introduction In this experiment, the genomic DNA of E. coli cells were isolated, purified, and quantified. To achieve this, E. coli cells were lysed, purified from impurities and Methods E. coli cells were first centrifuged in order to separate the cell pellet from the supernatant. Cell pellets were resuspended in saline-EDTA, which weakens bacterial membranes and chelates metal ions. Since E. coli has three layers (outer membrane,…

The orthologs of candidate genes in QTL regions and their annotation in Arabidopsis were obtained from the result of BLASTn based on gene sequence. Some genes related to seed weight from papers and some searched from Arabidopsis database (https://www.arabidopsis.org/index.jsp) using key words “seed weight”, “seed size”, “cell number” and “endosperm growth”. Homologues were clustered and analyzed using the Database for Annotation Visualization and Integrated Discovery (DAVID) tool, an online…

career; however, the opportunity to discover new information through research is one of my most intellectually stimulating experiences. With my goal to learn as much as possible under Dr. Nolan Kane, the Professional Investigator of the Cannabis Genomic Research Initiative (CGRI), I have expanded my traditional bench lab experience to bioinformatics, hemp breeding, and leading designing and performing my own project examining differential protein expression in Cannabis. Although I am deeply…

in Science, he talked about the ability to use genomic knowledge and apply it to the pharmaceutical field. Anne Fausto-Sterling’s The Bare Bones of Race talked about, the racial difference in bone density and uses and definitions of race in medicine. Joan H. Fujimura, Troy Duster, and Ramya Rajagopalan’s collective piece, Special Issue on race, Genomics, and Biomedicine, talked about the issues of studies of science and debated concerning race genomics, and diseases. The first reading, Race…

social science theories during implementation of care. These three theories are family systems, family development and family bioecological systems. Some families share the same genes which has caused a rise in examining genomic health. This is an analysis of family theories and genomic health. According to Stanhope & Lancaster, 2014 the family system operates as a whole verses an individual. The family maintains stability by adapting to stressors together. When one family member has an illness,…

veteran that became a biotechnology icon (Cold Spring Harbor Laboratory, 2003). His works have contributed enormously to biotechnology. His contributions include: Celera’s Human Genome Project, the creation of the first synthetic genome, and Synthetic Genomics, by which he proves that biotechnological breakthroughs can be accomplished in the private sector of research. Not only did his own work…

further researched about the chain of Ebola virus infection, to describe the determinants involved in the EVD spread, and to discuss the susceptibility of outbreaks in developing countries. Researchers are only beginning to investigate the virus genomics, the transmission in humans, and epidemiological features unique to the virus. This is because most of this information remains incomplete due to limited data collection before the 2014 epidemic. Multiple hypotheses have been proposed regarding…

Genomic engineering is the process of modifying specific sites in DNA sequences of cells or organisms. The ability to achieve effective and precise editing of the genome has been achieved and advanced through the development of sequence-specific nucleases1. Zinc-finger nucleases and transcription activator-like effector nucleases (TALENs) are programmable DNA binding nucleases previously used to achieve this targeted editing of DNA. However these tools have been found to be both time consuming…

We are in a new era of healthcare thanks to the advancement of genomics, advances in eHealth technologies and the increased ability to share knowledge via clinical information systems. Coupled with the increase in aging population, upsurge of chronic disease and major shifts in recent health care policy, the definition of “personalized care” is vastly expanding and diversifying. The antiquated “one size fits all” prescribing and treatment methods of current day medicine are no longer adequate.…