The same reaction mixture without genomic DNA was set up to serve as a negative control. The PCR reactions were then amplified in a DNA thermocycler (G-Storm GS1 thermal cycler, GRI Ltd., Gene House, Essex, United Kingdom) programmed as follows: (1) 94°C for 2 min (initial denaturation); (2) 94°C for 1 min (denaturation), (3) 56°C (in the case of Ol12-E03 primer) or 52°C (in the case of for Ol12-F11, Ol10-H02, Ol10-A03a, Ol10-F11a primers)…
is known in the medical field as genetically engineered of human gametes, zygotes and embryos also known as germ line modification, (Wikipedia). There are countless problems surrounding designer babies, an example being a gene does not code just for one function, thus a gene medication can be used to solve a problem while it causes another problem involuntarily, (world press 2013, April 28). People have questions about this process such as; is designing a baby ethical? Then there are problems…
Fertility Preservation is a field in Fertility that was created by Dr. Kutluk Oktay. Fertility Preservation is the process of freezing reproductive tissue to be used later in the making of children. Fertility Preservation can be used to help couples that can’t have children, have children. People with cancer or other diseases that would disable themselves to be able to have children, now can because of Fertility Preservation. It can change the course of somebodies life. Dr. Oktay is very happy…
Results Primer design and LAMP reaction Among 40 different primers (OPA 1-20 & OPB 1-20 series) screened by RAPD PCR for differentiating the Mycosphaerella eumusae from other leaf spot causing fungal pathogens in banana, only the primer OPB 10 has generated a unique amplicon specific to M. eumusae at 1300 bp size. A total of nine different sets of LAMP primers were designed based on this specific SCAR derived RAPD marker sequence of M. eumusae and screened for the production of LAMP products…
such as genetic engineering, recombinant DNA technology, tissue culture, protoplast culture etc. has helped to produce improved quality crops. The use of chemical fertilizers can only increase the production but research on genetic level to identify genes can not only improve quality but quantity of crops too. Food crops affected from insects and microbes can be treated using the r-DNA technology. Also there has been development to produce plants in the lab inside a cultural flask. Any part of…
HUMAN CLONING: PROS AND CONS OF REPRODUCTIVE CLONING Cloning is the process of creating identical cells, genes, organs or even organisms through asexual reproduction. It has been a common practice of mankind for a long time. It has becomes the debatable issue since it was used to clone animals around 40-50 years ago. Human cloning has created an offense toward ethical issue of human right. According to Yadav and Sharma (2011), the person who promoted human cloning must convince others and show…
Imagine hypothetical situation: two people are supposed to describe the color green. One of them speaks about the color of moss. The other about apples. What determines what’s going to be the first, intuitive response? Social background. No mater the effort, every human is bound by the way of thinking of his native society. The liberal education, although aspiring to the noble goals of wisdom and equality, is a victim to its social background as well. Therefore, the social bias, present in the…
CHAPTER-2 VARIABILITY AMONG THE ALTERNARIA BRASSICAE ISOLATES INTRODUCTION The chapter is mainly concerned with examining the variability among Alternaria brassicae isolates from the oilseed brassicas. Since, the oilseed brassicas are more prone to the fungal attack and thereby, resulting in reduced productivity of the oilseed. Therefore, the present investigation focuses on the studying the features such as morphological, pathological and molecular variability of Alternaria brassicae. The…
Biofuel from GM Macro algae Hydrocarbon biofuels are produced by genetically modified seaweed which are obtained by inserting genes from high hydrocarbon producing micro algae into high growth seaweed species. Botryococcus braunii (BB) a green micro algae that produces large amounts of aliphatic hydrocarbon molecules is used as a source for genetic material. The genes that produce hydrocarbon in BB are identified, removed, cloned and subsequently inserted into high growth brown seaweeds like…
3. Results Considering the morphological characteristics of the ecotypes, the cladode shape was elliptic or ovate, the number of spines varied among three classes (many, intermediate or few), the flowers had two types of petal color (scarlet or yellow), the fruit was elliptic or ovoid and the pulp color was white, pale yellow or orange (Table 2). The OFI populations studied showed statistically significant differences for DM of the fruits, F (17, 34.24) = 31.61, p < 0.05, which varied…