Gene expression

and differentiation of normal and leukemic cells of the hematopoietic, or white blood cell lineage (Xie et al., 1998). HL-60 cells are invaluable as they are easy to maintain, differentiate and can be used to investigate cell development, or gene expression. HL-60 cells can be used to induce differentiation via signal transduction into monocytes, granulocytes, and lymphocytes using various chemical agents (Cirtain et al., 2016). HL-60 cells are also used to test differentiation by viewing the…

Title: Construction of FATTY ACID DESATURASE 2 (FAD2) gene knockouts using RNAi novel technique in the model plant Thlaspi arvense. Abstract: FATTY ACID DESATURASE 2 and FATTYU ACID ELONGASE 1 (FAE1) are the two genes of interest present in the plant pennycress. FAD2 is in the pathway that synthesizes the polyunsaturated linoleic (18:2) and linoleic (18:3) fatty acids from the monounsaturated oleic acid (18:1). Polyunsaturated fatty acids are undesirable in biodiesel because they confer…

Although multiple AlkB homologue genes have been identified in mammalian genome, their function and substrates have been elusive. Zheng et al. demonstrated that human ALKBH5 protein efficiently catalyzes demethylation of m6A containing RNAs. Utilization of iron by ALKBH5 protein during…

strategy: To experimentally validate computationally predicted drivers of CRPC, we will use loss- and gain-of-function approaches for in vitro (i.e., cell lines) and in vivo (i.e., xenograft models) experimental validation to determine whether these genes are essential for drug-resistance. Computationally inferred drug combinations will be validated for their effect on MRs’ activity and ability to govern drug resistance and tumorigenicity. Furthermore, inferred drug combinations will be analyzed…

signaling is very active in metastatic breast cancer cells that express a specific gene. Wnt/B catenin signaling is prevalent in breast cancer cells that express micro RNA 374a. The micro RNA 374a directly targets and suppresses the multiple negative regulators of the Wnt-B catenin signaling such as WIF1, PTEN, and WNT5A. The inhibition of B-catenin signaling by the DKK1 or the SFRP1 protein overexpression reduces the expression of breast epithelial markers. An accumulation of B-catenin in the…

For years I fell under the falsified vision of wanting to become a doctor. I anticipated myself studying for the MCATs, attending medical school interviews, and fretting over my application until it was perfect in every way shape and form. Why a doctor? The answer was simple, I longed to be the person who could make a difference in not only an ailing individual, but to the world of medicine. I hoped to aid in finding cures to the worlds most complex of diseases and genetic mutations. I was…

Materials and Methods: This study was carried out at a lab in which involved numerous steps and experimental procedures. The overexpression and reduction of expression of the DJ-1 protein was acted upon normal endometrial cells and endometriotic cells through utilization of siRNA was studied. For the purpose of this experiment, several reagents were used such as: DMEM, FCS, antibiotics, and phenol red free DMEM…

GMO Labeling- The Argument against Labeling The labeling of Genetically Modified Organism is nearly as controversial as GMO’s themselves. Well-intentioned consumer groups have lobbied to have GMO products labeled to protect consumers. These groups argue that GMO’s may have the potential to be dangerous and should be labeled. Those that advise against labeling, industry, academics, and regulatory bodies sight the complexities and cost of labels that do not improve safety. Mankind has been…

will only become more problematic. (B.M. Chassy, 2007) Additionally, there is a huge risk of the unknown of what GMOs can cause. The main issue of concern, as outlined by the World Health Organization (WHO), is allergenicity, caused by the mixing of genes (WHO, 2001).…

The over expression of LacZ produced not a normal sized wing and eye acting as a control (Figure 4I and 4O). The over expression of both Pix and Git did not show any noticeable increase or decrease in the size of the fly eye or wing (Figure 4J and 4P). There was a slight decrease in wing size but the fly eye seemed to be normal when the overexpression of LacZ was paired with Hpo (Figure 4K and 4Q). The most noticeable affect occurred when there was an over expression of Hpo, Pix, and Git…