Gel electrophoresis

molecular techniques important for biologists to understand. In this portion of the lab, the chromosomal DNA (chDNA) will be isolated. Its purity will be measured using spectrophotometric analysis. Lastly, the DNA will be digested and verified via gel electrophoresis. A. fischeri is a gram negative rod shaped marine bacteria that exhibits bioluminescence and is both found free-living or in symbiosis. It has a variety of hosts such as the bobtail squid and is named after the German scientist…

glycoside bonds within the double stranded DNA, to create a single stranded DNA with a radioactive label on the 5’ end. The labeled substrate is then cleaved to create known endpoints and these fragments are run on a polyacrylamide gel using electrophoresis. The gel would then use…

Neutral community theory, also known as the unified neutral theory of biodiversity and biogeography was set-forth in literature in 2001 by Stephen Hubbell however it draws largely upon pre-established, and widely accepted, ecological theories of island biogeography (MacArthur and Wilson, 1967). The original model proposed by MacArthur and Wilson was constructed to account for variation in the composition of birds found in different sized areas as well as their relative abundance and…

better in rich and complex media, indicates that the organisms were in majority heterotrophs. PCR was done on all the colonies in the master plates in several occasions, both with 16s and 18s primers, but when the samples were analyzed through gel electrophoresis, no bands appeared, therefore the PCR samples from the culture dependent organisms were not sequenced. Although sequences for these organisms were not obtained, the samples were Gram stained in order to observe the morphological and…

creating a perfect product is the research being done on recombinant DNA. Recombinant DNA is any single molecule containing DNA sequences from two or more organisms. The process of creating recombinant DNA relies on the use of restriction enzymes, gel electrophoresis, and DNA ligase. The first step in creating this new DNA strand is to cut the gene sequences you want from the original DNA molecule. This sequence will be cut by the restriction enzyme in such a way that it creates a palindrome…

Introduction Charles Darwin is famous for his book, The Origin of Species, where he proposed that evolutionary change in populations is due to natural selection. His idea was that of survival of the fittest. In other words, the species with superior traits would have more of a likelihood to live, and then breed more offspring. Slowly, more and more of the species with the superior traits will survive and multiply. But what is it that gives these species the better survival trait, and allows…

A DNA ladder is a solution of DNA molecules of different but known length. In Well 1, the DNA ladder was applied to the gel to set a standard for the rest of the samples. In LoggerPro, after setting an origin, the second step was to set a standard ladder. We standardized the ladder by going from 4 to 5 cm and determining that was 10 milliliters. The next step was to scale the ladder by selecting the 3 brightest points and labeling it 3000,1000,500 in descending order. The estimated size of my…

David Daige Biol 1030H Due Date: April 1, 2016 Professor Laura Pacey Lab Report 5: Molecular Biology Techniques Abstarct The aim of this study is to accurately measure small volumes using the micropipettes and to use virtual STR and PCR programs to test the paternity of various cases. During the lab, 18uL of different volumes were successfully measured into a tubule using the micropipette. During the lab, fruit juice was accurately extracted from a solution using…

DNA regions with short and repeating units is called STR. These STRs are located around and surrounding the chromosomal centromere. STR analysis is a process containing three processes which are amplification, electrophoresis, and interpretation. In the first stage, amplification, DNA is extracted and added to chemical reagents which are then heated, which causes the two strands of DNA to separate. Reagents in which the sampling DNA is heated by contains markers, or…

As shown by the gel electrophoresis my DNA is very clean because, as shown by the picture my uncut DNA has very crisp and clean band in the gel. I wanted this one clean band to show that my DNA is not pre-cut in any way and is pure. The gel also shows that my DNA was cut by Ban II Cla, Eco, Hae III, and Sal enzymes. The gel shows that in these columns the DNA became spread out, and looks more like a smear on the gel, rather than one crisp band like the uncut…