Gas chromatography

Chromatography Abstract: The purpose of this lab is to use chromatography to separate and purify organic compounds. There are many different types of chromatography but most depend on the principle of adsorption. The two important components of chromatography are the adsorbent and the eluent. Adsorbents are usually solid materials that will attract and adsorb the materials to be separated. The eluent is the solvent, which carries the materials to be separated through the adsorbent. After being…

pigments. Mark, on the other hand, believed that the same pigments were also located in the green portion even though they were not visible. In order to determine who was right, two tests were conducted. The first tested conducted was the paper chromatography test. In this test, samples of both the green portion of the leaf and stem of a beet were turned into liquid samples. Portions of the both samples were then added…

The goal of this experiment was to use the technique of thin layer chromatography (TLC) to identify the active analgesic ingredient of Advil and monitor the transesterification of methyl salicylate from acetylsalicylic acid. The synthesized methyl salicylate was then purified using acid-base chemistry and analyzed using H NMR spectroscopy. Identification of a proper mobile phase to separate common analgesics used in over-the-counter pain relievers was essential in being able to determine the…

According to Chemguide, Chromatography is a physical method that is used to separate a mixture of chemical substances into its components, which then can be closely analyzed. All forms of chromatography share this same principle. Here, a mobile phase travels through a stationary phase, carrying the components of the mixture with it. It is possible that the mobile phase and the stationary phase have similarities or differences that affect the overall interaction between each other and the…

Experiment 5 Synthesis and NMR Spectroscopy of ethyl cinnamate Introduction: Wittig reactions involve the stereoselective synthesis of olefins from phosphonium ylides and aldehydes or ketones, where the carbonyl bond is converted to an alkene double bond.1 The variety of suitable reagents and the relatively mild experimental conditions facilitate their industrial applications, such as the synthesis of epoxides, esters, carotenoids and vitamin A.2,3 Specifically, the ester ethyl cinnamate is used…

Slime is made by mixing together borax, glue, and water. Depending on the amount of borax added to the mixture will determine the density of the slime. For our experiment in class the independent variable was the borax. The dependent variable was slime. The control group was glue. It was important to keep the amount of glue, borax, and water constant throughout the experiment. In an experiment conducted in class it was hypothesized that the more borax added the denser the slime will be.…

Every substance reacts with force. Whether it breaks, splashes, or holds together. The science behind this experiment is the suspension. The suspension is the mixture of two substances. Where one is finely divided and dispersed in the other(SteveSpanglerScience,2015). The purpose of this experiment is to analyze the different states of viscosity, varying on the force applies to the solution. This mixture has two different states when force is being applied. When touched slowly, it is simple to…

The role of paper chromatography is to separate various and different chemicals/mixtures to show individual components within. If there are any individual components within the mixture, then they will move up the paper at different speeds and this will make the components separate. When carrying out the process, drawing an origin line near the bottom of one of the ends on the chromatography paper will be the starting point of the mixtures that you are trying to separate when they move up the…

Task 3 P1 When using thin layer chromatography (TLC) to test for purity of aspirin it is a quick and simple method. Firstly when using the TLC method you need to have a TLC sheet and mark on the sheet 0.5cm up on the sheet and draw a line, make sure you don’t press too hard on the paper as this will disrupt the absorbent on the paper. You need to make sure the samples have been marked on the paper so you don’t get mixed up and leave a gap between the aspirin samples so they don’t run together…

Thin Layer chromatography was also use to separate the mixture of fluorene and fluorenone. Thin layer chromatography is a useful separation method when the components of a mixture are in question. Analyzing what the mixture consist of can be efficiently determine by TLC by calculating Rf and comparing these values. For the separation and isolation of a mixtures components, this method is extensive and not as efficient at column chromatography. This conclusion is represented in the data gathered.…