Gas chromatography

1. Abstract In this study, reverse micellar extraction of asparaginase model system was carried out using CTAB/Iso-octane/hexanol/butanol system to optimize the forward and back extraction efficiency. By using Taguchi’s orthogonal array (OA), maximum forward extraction efficiency of 55.0%, 61.0% and 54% was achieved with an aqueous phase pH of 11.0, 150mM CTAB/Iso-octane and 0.1 M NaCl, respectively. Similarly, Taguchi’s orthogonal array (OA) was applied to optimize the pH of stripping phase,…

Schiff Bases: Schiff bases are amongst the important class of drugs containing imine or azoethine (-C=N-) moiety,have been obtained by the condensation of primary amines and carbonyl compounds.It was first time presented by the Hugo schiff (Cimerman et al, 2000). Schiff bases are one of the most important class of organic drugs widely used in variety of different fields such as analytical, biological, and inorganic chemistry.The schiff bases heve been designed to synthesize more and more novel…

During this experiment that was conducted in partners, the concentration of RNase H that was purified in the previous experiment #6 was determined and also the concentration of an unknown given solution using their absorbance values obtained from the spectroscopy apparatus. These concentrations were calculated using the equation of the line produce with the values of the concentration (x-axis) and corresponding absorbance (y-axis) of five protein bovine gamma globulin standard solutions. In…

Introduction 1.1 Polyurethane polyurethanes were first developed by Otto Bayer and coworkers in 1937. Shortly thereafter, Schollenberger and coworkers used formula modification to prepare elastomeric polyurethane products from Bayer's products that were like nylon or other engineering plastics in physical characteristics. PUs are widely used in coatings, flexible and rigid foams, elastomers, and composites. Reasons for the use of polyurethane coatings include: high performance characteristics…

The mixture was stirred, and a white precipitate of dicyclohexyl urea (DCU) was created after 15–30 min. The reaction was monitored by thin layer chromatography (TLC). After approximately 6–8 h, the precipitate was filtered and the filtrate was evaporated to dryness. The resulting residue was dissolved in acetone, filtered to additional remove any DCU, and evaporated once more. 10% sodium bicarbonate…

Table 1 Composition of Lansoprazole microparticles Formulation code Amount of drug(mg) Amount of polymer chitosan (mg) Drug polymer Ratio Tween 80 (%w/v) F1 100 100 1:1 1.25 F2 100 200 1:2 1.25 F3 100 300 1:3 1.25 F4 100 400 1:4 1.25 F5 100 500 1:5 1.25 F6 100 600 1:6 1.25 Characterization of Lansoprazole microparticle The microparticle will be characterized for following parameters: Percentage yield: After complete drying, the drug loaded microparticle was collected and weighed accurately…

Objective: In this laboratory experiment three objectives were sought after. These objectives were to first, create a separation scheme. Second, perform basic biochemical qualitative analytical tests. Lastly, identify the major components of milk. Introduction: The three goals in this experiment were desirable due to the fact that it gave knowledge on what is actually digested when milk is consumed. This is notably important to the children and adults that are lactose intolerant. 1 Many…

Objective The objective of the experiment was to determine the purity and components of a mixture of fluorescein and bromonitroindoline, as well as separate them, through the process of thin layer chromatography. Procedure Part A- Separation of Compounds by Thin Layer Chromatography • Three 100 mL beakers and watch glasses were cleaned in preparation for this experiment. Three silica gel plates were acquired from the instructor, and to each plate a line was drawn 5 mm from the edge of the…

Anion exchange chromatography: In anion exchange chromatography negatively charged ions will bind to the column. This will leave positively charged ions flushed through the column. By picking a pH of 6.5, protein X will flush through the column while protein Y and Z will stick to the sides of the column. Then by changing the pH to 8 it would make protein Z negatively charged and will be stuck in the column as protein Y is flushed to the bottom of the column. Cation exchange chromatography: This…

Purpose The purpose of this lab was to separate unknown compound 204, composed of an amine, a carboxylic acid, and a neutral component, into each of its three respective states. The unknown compound was separated using microscale separation of acidic, basic, and neutral components by liquid/liquid extraction. Liquid/liquid extraction is a very common method often used in work-ups and allows for isolation of neutral components using acid/base chemistry; this method is extremely helpful in…