4.2.3.5. Antioxidant assays in gastric tissue homogenate 4.2.3.5.1. Catalase activity Catalase activity in the gastric tissue was measured according to the procedures of Sinha (1972). One ml of homogenate solution was taken and mixed with 5 ml of phosphate buffer of pH 7.4. To this 4 ml of 0.2 M H2O2 was added and time noted. Exactly after 180 seconds of adding H2O2, a set of 1ml of reaction mixture from the above was taken and 2 ml dichromate acetic acid was added. It was then kept in a boiling…
Protein Separation Using Gel Filtration Chromatography Introduction: Chromatography techniques are one of the most useful methods use for the isolation or separation of macromolecule from a mixture. The most common used chromatography techniques are thin -layer chromatography (TLC), column chromatography, etc. The purpose of this lab exercise is using the gel filtration chromatography to separate different proteins from a mixture based on their size. Also, collecting elutes that run through…
Introduction: Photosynthesis is the process used by plants, algae and some bacteria, to use the energy from the sunlight to transform carbon dioxide and water into glucose and oxygen. The equation for photosynthesis is: carbon dioxide + water light energy glucose + oxygen. Chloroplasts are an organelle found in the leaves of green plants and are essential in photosynthesis as they are responsible for producing the glucose and storing the energy. Photosynthesis is vital for all life on…
Procedure: Before beginning each experiment, remove all clutter from the workstation and read the directions for the experiments thoroughly. Part 1: Warm slightly more than 300 mL of water to approximately 70 degrees Celsius. Measure out 150 mL of water into the coffee cup calorimeter. Take the temperature of this water in the coffee cup calorimeter once it has reached a steady temperature. Then, add an ice cube approximately the size of a large marshmallow. Stir the ice and water and measure…
This reaction involves very intricate and steps that are critical to understanding the concept of equilibrium as a whole. First, an important concept to understand when describing any reaction is the idea of collision probability. When we examine gas molecules in a 1 cm3 container, there can be billions of collisions per second between neighboring molecules, and even then, maybe only one of these collisions result in a reaction. If any one collision occurs, the only way they will react to form a…
quality. For the Marcet boiler experiment, it was found that the higher the system pressure, the higher the saturated temperature. For the Boyle’s law experiment, it was found that pressure is directly proportional to volume, despite using non-ideal gas for the experiment. 1 Introduction and Theory The aim of this lab demonstration is to get familiar with operating pressure systems in order to investigate…
Ultrasonic interferometer Working principle An ultrasonic interferometer is a simple and direct device to determine the ultrasonic velocity in liquids with a high degree of accuracy. An ultrasonic interferometer is used to determine the accurate ultrasonic velocity in liquids. The principle used in the measurement of velocity is based on the accurate determination of the wavelength in the medium. The principle of ultrasonic interferometer for the measurement of velocity is determination of the…
When dry ice is put into water they start to reacting and boiling. That reaction is called sublimation. Sublimation is when something solid goes straight to gas skipping the liquid phase. So because the water is warm it heats up the dry ice which causes it to form a gas. The gas is carbon dioxide. The temperatures of everything are very strange. The water cannot be cold when trying the experiment because it will not heat up the dry ice and create carbon dioxide gasses but it will probably…
A filter is something that removes impurities or solid particles from a liquid or gas that passes through it. There has been a total of 76% of deaths due to infections from dirty liquids and poisonous gases. Not many filters do the same job you would like it to do, but with this particular filter it is made to filter out gases, liquids, and solids from a variety of different elements and objects as well as cleaning any object you toss into it. The item's frame is powerful enough to do these…
A mixture of two unknown liquids were obtained. The assigned mixture code was recorded in the notebook (#10). The mixture of the two miscible liquids contained a low boiling point and high boiling point. The method used to separate the two miscible liquids was simple distillation and therefore a simple distillation apparatus was set up. The apparatus included 250mL distillation flask, a 100ml receiving flask, a condenser for water to run through, and a thermometer to monitor the temperature. The…