Full genome sequencing

It’s important to realize that each scientific break through is made possible by the work that came before it. It’s a lot like putting puzzle pieces together, collecting different important evidence until enough puzzle pieces result in another break through. Fifty years ago two scientists announced to a lunch time crowd that they had discovered the secret to life. How DNA changed the world To begin with scientists used to have no understanding of what caused distinct patterns of inheritance.…

1.1 Caenorhabditis elegans (C. elegans) Caenorhabditis elegans is a free-living non-parasitic, non-infectious, non-pathogenic, transparent Nematode (roundworm). It has a full grown length of about 1mm, a life span of around 3 weeks and is found in temperate soil environments where it survives by feeding on microbes such as bacteria. [1] Consisting of about 1000 somatic cells as an adult, C. elegans is amenable to genetic crosses and produces a large number of progeny, at times even…

“Genome editing” is a form of genetic engineering that involves the addition, deletion, and modification of genes in a genome. The genome of an organism is the full genetic complement of that given organism hence by editing the genome, scientists are able to alter the phenotype of these organisms and can modify the cellular functions occurring within the organism. Mutations in the DNA of an organism occur during DNA replication. For example in the human genome, it is thought that there are 6…

DNA plasmid was added to each of the “+plasmid” and it expresses the ampicillin resistance gene. According to the data, that didn’t happen because the +pGLO LB/amp had 3 white colonies at the end of the bacterial transformation and the +pGLO LB/amp/ara had 2 colonies that glow at the end of bacterial transformation meaning that there was an error in our “+plasmid” which occurred during the lab. We did predict that the -pGLO LB/amp would remain the same with no colonies and -pGLO LB would grow…

Virus Taxonomy Order: Unassigned Family: Bunyaviridae Genus: Phlebovirus Species: Toscana virus talk.ictvonline.org Structure and genome The toscana virus (TOSV) is a negative-single stranded RNA virus, it is spherical in shape, it has an enveloped surrounding its capsid, and it has a diameter of 80–120 nm. Its genome is partitioned into three segments, termed (large)L, (medium)M and (small)S. With L about 6,400 nucleotides in length, M about 4,200 nucleotides in…

Comparative Analysis of Plant Genome – An Overview Introduction Comparative genomics is used to study the similarities and differences in the structure and function of genome in living organisms. In the medical field, this information can help scientist to better understand the human genome. This allows them to develop new strategies to combat human disease. Comparative genomics is also a powerful tool to study the evolutionary relationship among organisms. We are able to identify genes that…

create another. Although CRISPER/CAS 9 is often precise, it has been known to cut the wrong strand of DNA leading to a whole new problem. The main subject of debate is whether or not to allow germ-line gene editing. Germ line editing is altering the genome in the sperm or egg cell. This provides the ability to change the genetics that a child is born with and these change s traits that would be inherited further down the generations. In altering the inherited traits we may be causing more…

Genetic transformation is the process in which a gene from one organism inserts itself into a different organism thus changing that organism’s genetic makeup, “Genetic transformation literally means change caused by genes and involves the insertion of a gene into an organism in order to change the organism’s trait.” Abraham, Anup.2016. Bacterial Transformation. Biology 281 Conceptual Approach to Biology for Majors I. Arizona State University. Hayden-McNeil. In this experiment, bacteria will be…

Kwon-Chung in 1976 has mentioned that C. neoformans exists as two mating types, MATa and MATα which is determined by single locus with diomorphic alleles.(Halliday CL et al, 1999). The MAT locus of C. neoformans is larger and consists of 100 kb having more than 20 conserved genes. These mating types play an important role in sexual development changes. These also play a vital role in epidemiology by knowing the prevalence and virulence of the isolate (Lengeler KB et al, 2000). Based on the…

In this globalization era with well-developed nations and advanced technologies, it happens where great minds combined with those technological advancements, have a lead to creations and achievements that somehow would have been impossible to be created or achieved. Genetic engineering made it possible to this matter. Generally, it is understood that genetic engineering itself is the technique of altering the genetic constitution of cells (or individuals) by the selective removal, insertion or…