Freedom of expression

3. Results 3.1 Cloning and phylogenetic analysis B. bigemina Argentina strain was used to obtain genomic and cDNA for PCR with primers BbigAMA-1 F and R to amplify the AMA-1 gene. The sequences had an identical length from both templates and of the estimated size according to primers' design (not shown). The nucleotide sequencing analysis presented a 1988-bp long gene (gene bank accession number: AB481200) and encoded a 595-amino acid peptide (gene bank accession number: BAH22706) (Fig. 1)…

The wide field of biochemistry includes exploration in the use of gene therapy for the treatment of cancers such as lung cancer. Though gene therapy is still in early stages of development, many researchers have concluded that gene therapy in therapeutic cancer treatment seems to be very effective and efficient. Gene therapy is the replacement of defective genes to enable the production of normally functioning genes. This replacement of genes is an adolescent and risky procedure and is often…

that are neither differentially expressed nor differentially active in castration-resistant tumors and are not regulated by any MR. Conversely, MRs whose activity decreases in castration-resistant state will be tested in over-expression essays using lentiviral cDNA expression…

all isoform, isoform abundance views, expression views, junction views are shown for selected tissue. In this view, the expression level shows as transcript per million (TPM) and the highest isoforms expressed in brain (blue) is ENST00000453943, heart (orange) is ENST00000312726 and liver (green) is ENST00000391865. The sashimi plot shows the quantitative visualisation of the RNA-seq read alignment of brain, heart and liver together with the gene expression shows in FPKM value (Figure 4.12C).…

Because she has so much going through her head constantly, she works to maintain a neutral face expression. In this scene alone she has about four different plots going she has to maintain. She works to stay “innocent” as Cecile laments about this horrific event, all engineered by Merteuil herself. One of my favorite moments of the scene however, is…

Gene expression within a eukaryotic cell is directly impacted by the spatial organization of the nucleus [1]. Cellular organization is carried out at the chromosome-level [1], through means of chromosome folding, gene kissing, looping, nuclear lamina associations and protein-nucleic acid interactions within nuclear bodies. Observation of the nucleolus using Raman microspectroscopy may provide more insight into macromolecule density and distribution, as well as provide answers to the still…

The world is constantly changing, therefore the way food is grown must change too. Companies are adding GMO’s or Genetically Modified Organisms to food without telling consumers. These Genetically Modified Organisms are used to help food grow and deal with the weather changes and parasites. Congress has passed a bill that requires companies to label products made with GMO’s. I think Congress made a smart decision to make companies label their products. People need to know what they are putting…

being heard, but also they can communicate other messages depending on the tone and intensity of voice and non-verbal behaviour accompanying them. When the content of what the client was saying was sad, I used a low tone of voice and sad facial expression to communicate my empathy to her (e.g. line 105, 108). One limitation in the use of minimal encouragers was a lack of variety. Varying minimal encouragers can be a subtle way of demonstrating that you are completely engaged in the client’s…

chemical expressed in female flowers, the gene expression changes from this treatment were not discussed (1991). What differential RNA expression occurs when transitioning female flowers to male flowers through CS treatment on Cannabis? My entire honor’s thesis will be performed in The Kane Lab using RNA donated from Skinny Pineapple LLC that will be prepared, sequenced, and analyzed on campus. This project will help better understand Cannabis’s gene expression patterns during vegetative…

Materials and Methods: This study was carried out at a lab in which involved numerous steps and experimental procedures. The overexpression and reduction of expression of the DJ-1 protein was acted upon normal endometrial cells and endometriotic cells through utilization of siRNA was studied. For the purpose of this experiment, several reagents were used such as: DMEM, FCS, antibiotics, and phenol red free DMEM…